Stability of spore-based biosensing systems under extreme conditions

Abhishek Sangal, Patrizia Pasini, Sylvia Daunert

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The full exploitation of bacterial whole-cell biosensing systems in field applications requires the survival of bacterial cells and long-term preservation of their sensing ability during transportation and on-site storage of such analytical systems. Specifically, there is a need for rapid, simple and inexpensive biosensing systems for monitoring human health and the environment in remote areas as well as developing countries, which often suffer from harsh atmospheric conditions and inadequate commercial distribution and storage facilities. Our laboratory has previously reported the successful use of bacterial spores as vehicles for the long-term preservation and storage of whole-cell biosensing systems at room temperature. In the present research, we have accomplished a year-long study to investigate the effect of extreme climatic conditions on the stability of spores-based whole-cell biosensing systems. The spores were stored in laboratory conditions that simulated those found in real harsh environments, including, extreme temperatures and humidity levels, and desiccation. This study was crucial in determining the germination ability and analytical performance of the spore-based sensing systems upon storage in such conditions in order to support their effective use in the field, in extreme environments. Our results proved that the intrinsic resistance of spores to harsh environmental conditions helped maintain the integrity of the encapsulated sensor bacteria. The revived active cells actually retained their analytical performance during the course of the 12-month storage study. We envision that spore-based sensing systems could pave the way to the use of whole-cell biosensors in field applications and in areas where they have not been employed so far.

Original languageEnglish
Pages (from-to)377-382
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume158
Issue number1
DOIs
StatePublished - Nov 15 2011

Fingerprint

spores
cells
Developing countries
Biosensors
Atmospheric humidity
Bacteria
Cells
Health
Temperature
Monitoring
Sensors
germination
meteorology
exploitation
bioinstrumentation
integrity
bacteria
drying
health
humidity

Keywords

  • Bacterial spores
  • Biosensor preservation
  • Biosensor storage
  • Extreme environmental conditions
  • On-site applications
  • Whole-cell biosensing systems

ASJC Scopus subject areas

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Stability of spore-based biosensing systems under extreme conditions. / Sangal, Abhishek; Pasini, Patrizia; Daunert, Sylvia.

In: Sensors and Actuators, B: Chemical, Vol. 158, No. 1, 15.11.2011, p. 377-382.

Research output: Contribution to journalArticle

Sangal, Abhishek ; Pasini, Patrizia ; Daunert, Sylvia. / Stability of spore-based biosensing systems under extreme conditions. In: Sensors and Actuators, B: Chemical. 2011 ; Vol. 158, No. 1. pp. 377-382.
@article{976be4e4536f448da032c2ee30fbb8e0,
title = "Stability of spore-based biosensing systems under extreme conditions",
abstract = "The full exploitation of bacterial whole-cell biosensing systems in field applications requires the survival of bacterial cells and long-term preservation of their sensing ability during transportation and on-site storage of such analytical systems. Specifically, there is a need for rapid, simple and inexpensive biosensing systems for monitoring human health and the environment in remote areas as well as developing countries, which often suffer from harsh atmospheric conditions and inadequate commercial distribution and storage facilities. Our laboratory has previously reported the successful use of bacterial spores as vehicles for the long-term preservation and storage of whole-cell biosensing systems at room temperature. In the present research, we have accomplished a year-long study to investigate the effect of extreme climatic conditions on the stability of spores-based whole-cell biosensing systems. The spores were stored in laboratory conditions that simulated those found in real harsh environments, including, extreme temperatures and humidity levels, and desiccation. This study was crucial in determining the germination ability and analytical performance of the spore-based sensing systems upon storage in such conditions in order to support their effective use in the field, in extreme environments. Our results proved that the intrinsic resistance of spores to harsh environmental conditions helped maintain the integrity of the encapsulated sensor bacteria. The revived active cells actually retained their analytical performance during the course of the 12-month storage study. We envision that spore-based sensing systems could pave the way to the use of whole-cell biosensors in field applications and in areas where they have not been employed so far.",
keywords = "Bacterial spores, Biosensor preservation, Biosensor storage, Extreme environmental conditions, On-site applications, Whole-cell biosensing systems",
author = "Abhishek Sangal and Patrizia Pasini and Sylvia Daunert",
year = "2011",
month = "11",
day = "15",
doi = "10.1016/j.snb.2011.06.039",
language = "English",
volume = "158",
pages = "377--382",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Stability of spore-based biosensing systems under extreme conditions

AU - Sangal, Abhishek

AU - Pasini, Patrizia

AU - Daunert, Sylvia

PY - 2011/11/15

Y1 - 2011/11/15

N2 - The full exploitation of bacterial whole-cell biosensing systems in field applications requires the survival of bacterial cells and long-term preservation of their sensing ability during transportation and on-site storage of such analytical systems. Specifically, there is a need for rapid, simple and inexpensive biosensing systems for monitoring human health and the environment in remote areas as well as developing countries, which often suffer from harsh atmospheric conditions and inadequate commercial distribution and storage facilities. Our laboratory has previously reported the successful use of bacterial spores as vehicles for the long-term preservation and storage of whole-cell biosensing systems at room temperature. In the present research, we have accomplished a year-long study to investigate the effect of extreme climatic conditions on the stability of spores-based whole-cell biosensing systems. The spores were stored in laboratory conditions that simulated those found in real harsh environments, including, extreme temperatures and humidity levels, and desiccation. This study was crucial in determining the germination ability and analytical performance of the spore-based sensing systems upon storage in such conditions in order to support their effective use in the field, in extreme environments. Our results proved that the intrinsic resistance of spores to harsh environmental conditions helped maintain the integrity of the encapsulated sensor bacteria. The revived active cells actually retained their analytical performance during the course of the 12-month storage study. We envision that spore-based sensing systems could pave the way to the use of whole-cell biosensors in field applications and in areas where they have not been employed so far.

AB - The full exploitation of bacterial whole-cell biosensing systems in field applications requires the survival of bacterial cells and long-term preservation of their sensing ability during transportation and on-site storage of such analytical systems. Specifically, there is a need for rapid, simple and inexpensive biosensing systems for monitoring human health and the environment in remote areas as well as developing countries, which often suffer from harsh atmospheric conditions and inadequate commercial distribution and storage facilities. Our laboratory has previously reported the successful use of bacterial spores as vehicles for the long-term preservation and storage of whole-cell biosensing systems at room temperature. In the present research, we have accomplished a year-long study to investigate the effect of extreme climatic conditions on the stability of spores-based whole-cell biosensing systems. The spores were stored in laboratory conditions that simulated those found in real harsh environments, including, extreme temperatures and humidity levels, and desiccation. This study was crucial in determining the germination ability and analytical performance of the spore-based sensing systems upon storage in such conditions in order to support their effective use in the field, in extreme environments. Our results proved that the intrinsic resistance of spores to harsh environmental conditions helped maintain the integrity of the encapsulated sensor bacteria. The revived active cells actually retained their analytical performance during the course of the 12-month storage study. We envision that spore-based sensing systems could pave the way to the use of whole-cell biosensors in field applications and in areas where they have not been employed so far.

KW - Bacterial spores

KW - Biosensor preservation

KW - Biosensor storage

KW - Extreme environmental conditions

KW - On-site applications

KW - Whole-cell biosensing systems

UR - http://www.scopus.com/inward/record.url?scp=79960469755&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960469755&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2011.06.039

DO - 10.1016/j.snb.2011.06.039

M3 - Article

AN - SCOPUS:79960469755

VL - 158

SP - 377

EP - 382

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

IS - 1

ER -