Src family kinase involvement in muscarinic receptor-induced tyrosine phosphorylation in differentiated SH-SY5Y cells

Piyajit Watcharasit, Janusz Tucholski, Richard S Jope

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Muscarinic receptor-mediated changes in protein tyrosine phosphorylation were examined in differentiated human neuroblastoma SH-SY5Y cells. Treatment of differentiated cells with 1 mM carbachol caused rapid increases in the tyrosine phosphorylation of focal adhesion kinase (FAK), Cas, and paxillin. The src family kinase-selective inhibitor PP1 reduced carbachol-stimulated tyrosine phosphorylation of FAK, Cas, and paxillin by 50 to 75%. In contrast, carbachol-stimulated activation of ERK1/2 was unaffected by PP1. Src family kinase activation by carbachol was further demonstrated by increased carbachol-induced tyrosine phosphorylation of the src-substrate, p120, and tyrosine phosphorylation of the src family kinase activation-associated autophosphorylation site. Site-specific FAK phosphotyrosine antibodies were used to determine that the carbachol-stimulated increase in the autophosphorylation of FAK was unaffected by pretreatment with PP1, whereas the carbachol-stimulated increase in the src family kinase-mediated phosphotyrosine of FAK was completely blocked by pretreatment with PP1. In SH-SY5Y cell lines stably overexpressing Fyn, the phosphotyrosine immunoreactivity of FAK was 625% that of control cells. Thus, muscarinic receptors activate protein tyrosine phosphorylation in differentiated cells, and the tyrosine phosphorylation of FAK, Cas, and paxillin, but not ERK1/2, is mediated by a src family tyrosine kinase activated in response to stimulation of muscarinic receptors.

Original languageEnglish
Pages (from-to)809-816
Number of pages8
JournalNeurochemical Research
Volume26
Issue number7
DOIs
StatePublished - Sep 26 2001
Externally publishedYes

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Focal Adhesion Protein-Tyrosine Kinases
Phosphorylation
src-Family Kinases
Carbachol
Muscarinic Receptors
Tyrosine
Paxillin
Phosphotyrosine
Chemical activation
Neuroblastoma
Proteins
Cells
Cell Line
Antibodies
Substrates

Keywords

  • Focal adhesion kinase
  • Fyn
  • Muscarinic receptor
  • Paxillin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Src family kinase involvement in muscarinic receptor-induced tyrosine phosphorylation in differentiated SH-SY5Y cells. / Watcharasit, Piyajit; Tucholski, Janusz; Jope, Richard S.

In: Neurochemical Research, Vol. 26, No. 7, 26.09.2001, p. 809-816.

Research output: Contribution to journalArticle

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