src- and fps-containing avian sarcoma viruses transform chicken erythroid cells

P. Kahn, Rebecca D Adkins, H. Beug, T. Graf

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We report here that several oncogene-transducing avian sarcoma virus strains, namely Rous sarcoma virus (src), Fujinami sarcoma virus (fps), and PRCII (fps), transform avian erythroid cells in vitro and in vivo. The src- and fps-transformed erythroblasts grow in vitro for 20-30 generations, require special growth conditions, and tend to differentiate spontaneously. In these properties, they resemble erythroid cells transformed with the erbB-containing H strain of avian erythroblastosis virus (AEV-H) but differ from those transformed with AEV-ES4 (erbA, erbB), which grow under standard culture conditions and rarely differentiate spontaneously. Erythroblasts transformed with viruses carrying temperature-sensitive mutations in the src or fps oncogene and then shifted to the nonpermissive temperature in the presence of anemic serum (as a source of an erythropoietin-like factor) differentiate terminally into erythrocytes. These results demonstrate that several members of the src gene family other than erbB have the capacity to transform erythroid cells.

Original languageEnglish
Pages (from-to)7122-7126
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number22 I
StatePublished - Dec 1 1984
Externally publishedYes

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Avian Sarcoma Viruses
Erythroid Cells
Sarcoma
Chickens
Viruses
Erythroblasts
Oncogenes
Alpharetrovirus
Rous sarcoma virus
Temperature
Erythropoietin
Erythrocytes
Mutation
Growth
Serum
Genes

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

src- and fps-containing avian sarcoma viruses transform chicken erythroid cells. / Kahn, P.; Adkins, Rebecca D; Beug, H.; Graf, T.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, No. 22 I, 01.12.1984, p. 7122-7126.

Research output: Contribution to journalArticle

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