src- and fps-containing avian sarcoma viruses transform chicken erythroid cells

P. Kahn, B. Adkins, H. Beug, T. Graf

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34 Scopus citations


We report here that several oncogene-transducing avian sarcoma virus strains, namely Rous sarcoma virus (src), Fujinami sarcoma virus (fps), and PRCII (fps), transform avian erythroid cells in vitro and in vivo. The src- and fps-transformed erythroblasts grow in vitro for 20-30 generations, require special growth conditions, and tend to differentiate spontaneously. In these properties, they resemble erythroid cells transformed with the erbB-containing H strain of avian erythroblastosis virus (AEV-H) but differ from those transformed with AEV-ES4 (erbA, erbB), which grow under standard culture conditions and rarely differentiate spontaneously. Erythroblasts transformed with viruses carrying temperature-sensitive mutations in the src or fps oncogene and then shifted to the nonpermissive temperature in the presence of anemic serum (as a source of an erythropoietin-like factor) differentiate terminally into erythrocytes. These results demonstrate that several members of the src gene family other than erbB have the capacity to transform erythroid cells.

Original languageEnglish (US)
Pages (from-to)7122-7126
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number22 I
StatePublished - 1984
Externally publishedYes

ASJC Scopus subject areas

  • General


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