Sporozoite loads of naturally infected Anopheles in Kilifi district, Kenya

Ephantus W. Kabiru, Charles M. Mbogo, Samuel K. Muiruri, John H. Ouma, John I. Githure, John C. Beier

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The number of salivary gland malaria sporozoites (sporozoite load) was determined by hemacytometer counts for 2,055 field-collected Anopheles mosquitoes from Kilifi District, Kenya. Of 48 gland-positive Anopheles gambiae s.l., sporozoite loads ranged from 125 to 79,875, with a geometric mean of 1,743 sporozoites per infected mosquito. About half of the infected mosquitoes had sporozoite loads <1,000. Following hemacytometer examination of salivary gland samples, the same samples were subsequently tested for Plasmodium falciparum circumsporozoite (CS) protein by enzyme-linked immunosorbent assay (ELISA). The confirmation by ELISA of CS protein in 89.6% (43/48) of the salivary gland-positive samples compared to only 1.4% (28/ 2,007) of the dissection-negative mosquitoes indicated that dissection methods with hemacytometer counts of sporozoites were adequate for detecting even low numbers of sporozoites in field-collected mosquitoes. Detection of 17 or fewer sporozoites in blood meals of 7 freshly bloodfed An. gambiae s.l. provides a further indication that the actual number of sporozoites transmitted during bloodfeeding may be quite low.

Original languageEnglish (US)
Pages (from-to)259-262
Number of pages4
JournalJournal of the American Mosquito Control Association
Volume13
Issue number3
StatePublished - Sep 1 1997
Externally publishedYes

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Public Health, Environmental and Occupational Health
  • Insect Science

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    Kabiru, E. W., Mbogo, C. M., Muiruri, S. K., Ouma, J. H., Githure, J. I., & Beier, J. C. (1997). Sporozoite loads of naturally infected Anopheles in Kilifi district, Kenya. Journal of the American Mosquito Control Association, 13(3), 259-262.