Spontaneous axonal regeneration in rodent spinal cord after ischemic injury

M. Von Euler, A. M. Janson, J. O. Larsen, Å Seiger, L. Forno, Mary B Bunge, E. Sundström

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Here we present evidence for spontaneous and long-lasting regeneration of CNS axons after spinal cord lesions in adult rats. The length of 200 kD neurofilament (NF)-immunolabeled axons was estimated after photochemically induced ischemic spinal cord lesions using a stereological tool. The total length of all NF-immunolabeled axons within the lesion cavities was increased 6- to 10-fold at 5, 10, and 15 wk post-lesion compared with 1 wk post-surgery. In ultrastructural studies we found the putatively regenerating axons within the lesion to be associated either with oligodendrocytes or Schwann cells, while other fibers were unmyelinated. Immunohistochemistry demonstrated that some of the regenerated fibers were tyrosine hydroxylase- or serotonin-immunoreactive, indicating a central origin. These findings suggest that there is a considerable amount of spontaneous regeneration after spinal cord lesions in rodents and that the fibers remain several months after injury. The findings of tyrosine hydroxylase- and serotonin-immunoreactivity in the axons suggest that descending central fibers contribute to this endogenous repair of ischemic spinal cord injury.

Original languageEnglish
Pages (from-to)64-75
Number of pages12
JournalJournal of Neuropathology and Experimental Neurology
Volume61
Issue number1
StatePublished - Jan 12 2002
Externally publishedYes

Fingerprint

Axons
Regeneration
Rodentia
Spinal Cord
Wounds and Injuries
Tyrosine 3-Monooxygenase
Serotonin
Spinal Cord Regeneration
Intermediate Filaments
Schwann Cells
Oligodendroglia
Spinal Cord Injuries
Immunohistochemistry

Keywords

  • Axon
  • CNS
  • Global spatial sampling
  • Ischemia
  • Neurofilament
  • Regeneration
  • Spinal cord injury

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neuroscience(all)

Cite this

Von Euler, M., Janson, A. M., Larsen, J. O., Seiger, Å., Forno, L., Bunge, M. B., & Sundström, E. (2002). Spontaneous axonal regeneration in rodent spinal cord after ischemic injury. Journal of Neuropathology and Experimental Neurology, 61(1), 64-75.

Spontaneous axonal regeneration in rodent spinal cord after ischemic injury. / Von Euler, M.; Janson, A. M.; Larsen, J. O.; Seiger, Å; Forno, L.; Bunge, Mary B; Sundström, E.

In: Journal of Neuropathology and Experimental Neurology, Vol. 61, No. 1, 12.01.2002, p. 64-75.

Research output: Contribution to journalArticle

Von Euler, M, Janson, AM, Larsen, JO, Seiger, Å, Forno, L, Bunge, MB & Sundström, E 2002, 'Spontaneous axonal regeneration in rodent spinal cord after ischemic injury', Journal of Neuropathology and Experimental Neurology, vol. 61, no. 1, pp. 64-75.
Von Euler M, Janson AM, Larsen JO, Seiger Å, Forno L, Bunge MB et al. Spontaneous axonal regeneration in rodent spinal cord after ischemic injury. Journal of Neuropathology and Experimental Neurology. 2002 Jan 12;61(1):64-75.
Von Euler, M. ; Janson, A. M. ; Larsen, J. O. ; Seiger, Å ; Forno, L. ; Bunge, Mary B ; Sundström, E. / Spontaneous axonal regeneration in rodent spinal cord after ischemic injury. In: Journal of Neuropathology and Experimental Neurology. 2002 ; Vol. 61, No. 1. pp. 64-75.
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