Splenic eumelanin differs from hair eumelanin in C57BL/6 mice

Przemyslaw M. Plonka, Dominika Michalczyk, Malgorzata Popik, Bori Handjiski, Andrzej Slominski, Ralf Paus

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The presence of melanin in spleens of black C57BL/6 mice has been known for long. Although its origin and biological functions are still obscure, the relation of splenic melanin to the hair follicle and skin pigmentation was suggested. Here, we demonstrated using for the first time electron paramagnetic resonance spectroscopy that black-spotted C57BL/6 spleens contain eumelanin. Its presence here is a "yes or no" phenomenon, as even in the groups which revealed the highest percentage of spots single organs completely devoid of the pigment were found. Percentage of the spotted spleens decreased, however, with the progress of telogen after spontaneously-induced hair growth. The paramagnetic properties of the spleen eumelanin differed from the hair shaft or anagen VI skin melanin. The splenic melanin revealed narrower signal, and its microwave power saturability betrayed more heterogenous population of paramagnetic centres than in the skin or hair shaft pigment. Interestingly, the pigment of dry hair shafts and of the wet tissue of depilated anagen VI skin revealed almost identical properties. The properties of splenic melanin better resembled the synthetic dopa melanin (water suspension, and to a lesser degree - powder sample) than the skin/hair melanin. All these findings may indicate a limited degradation of splenic melanin as compared to the skin/hair pigment. The splenic eumelanin may at least in part originate from the skin melanin phagocyted in catagen by the Langerhans cells or macrophages and transported to the organ.

Original languageEnglish (US)
Pages (from-to)433-441
Number of pages9
JournalActa Biochimica Polonica
Volume52
Issue number2
StatePublished - Aug 23 2005
Externally publishedYes

Fingerprint

Melanins
Inbred C57BL Mouse
Hair
Skin
Pigments
Spleen
Skin Pigmentation
eumelanin
Dihydroxyphenylalanine
Langerhans Cells
Hair Follicle
Macrophages
Electron Spin Resonance Spectroscopy
Microwaves
Powders
Paramagnetic resonance
Spectrum Analysis
Suspensions
Spectroscopy
Tissue

Keywords

  • EPR
  • Hair cycle
  • Hair follicle
  • Melanin degradation
  • Pigmentation
  • Spin-lattice relaxation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Plonka, P. M., Michalczyk, D., Popik, M., Handjiski, B., Slominski, A., & Paus, R. (2005). Splenic eumelanin differs from hair eumelanin in C57BL/6 mice. Acta Biochimica Polonica, 52(2), 433-441.

Splenic eumelanin differs from hair eumelanin in C57BL/6 mice. / Plonka, Przemyslaw M.; Michalczyk, Dominika; Popik, Malgorzata; Handjiski, Bori; Slominski, Andrzej; Paus, Ralf.

In: Acta Biochimica Polonica, Vol. 52, No. 2, 23.08.2005, p. 433-441.

Research output: Contribution to journalArticle

Plonka, PM, Michalczyk, D, Popik, M, Handjiski, B, Slominski, A & Paus, R 2005, 'Splenic eumelanin differs from hair eumelanin in C57BL/6 mice', Acta Biochimica Polonica, vol. 52, no. 2, pp. 433-441.
Plonka PM, Michalczyk D, Popik M, Handjiski B, Slominski A, Paus R. Splenic eumelanin differs from hair eumelanin in C57BL/6 mice. Acta Biochimica Polonica. 2005 Aug 23;52(2):433-441.
Plonka, Przemyslaw M. ; Michalczyk, Dominika ; Popik, Malgorzata ; Handjiski, Bori ; Slominski, Andrzej ; Paus, Ralf. / Splenic eumelanin differs from hair eumelanin in C57BL/6 mice. In: Acta Biochimica Polonica. 2005 ; Vol. 52, No. 2. pp. 433-441.
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