Spinal cord injury induces expression of RGS7 in microglia/macrophages in rats

Oliver N. Hausmann, Wen Hui Hu, Tal Keren-Raifman, D. Scott Witherow, Qiang Wang, Konstantin Levay, Beata Frydel, Vladlen Z Slepak, John R. Bethea

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

RGS proteins regulate G protein-mediated signalling pathways through direct interaction with the Gα subunits and facilitation of GTP hydrolysis. An RGS subfamily consisting of RGS 6, 7, 9, and 11 also interacts with the G protein β subunit Gβ5 via a characteristic Gγ-like domain. Thus far, these complexes were found only in neurons, with RGS7 being the most widely distributed in the brain. Here we confirm the expression of RGS7 in spinal neurons and show as a novel finding that following an experimental spinal cord injury in rats, expression of RGS7 is induced in a subpopulation of other cells. Immunofluorescent confocal microscopy using a series of cell specific antibodies identified these RGS7 positive cells as activated microglia and/or invading peripheral macrophages. To rule out interference from the adjacent neurons and confirm the presence of RGS7-Gβ5 complex in inflammatory cells, we performed immunocytochemistry, RT-PCR, Western blot, and immunoprecipitation using microglial (BV2) and peripheral macrophage (RAW) cell lines. Expression of RGS7 mRNA and protein are nearly undetectable in non-stimulated BV2 and RAW cells, but remarkably increased after stimulation with LPS or TNF-α In addition, RGS7-positive cells were also found in the perinodular rim in the rat spleen. Our findings show that RGS7-Gβ5 complex is expressed in immunocompetent cells such as resident microglia and peripheral macrophages following spinal cord injury. This expression might contribute to the post-traumatic inflammatory responses in the central nervous system.

Original languageEnglish
Pages (from-to)602-612
Number of pages11
JournalEuropean Journal of Neuroscience
Volume15
Issue number4
DOIs
StatePublished - Dec 31 2002

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Microglia
Spinal Cord Injuries
Macrophages
GTP-Binding Proteins
Neurons
RGS Proteins
Protein Subunits
Guanosine Triphosphate
Immunoprecipitation
Confocal Microscopy
Hydrolysis
Spleen
Central Nervous System
Western Blotting
Immunohistochemistry
Cell Line
Polymerase Chain Reaction
Messenger RNA
Antibodies
Brain

Keywords

  • CNS
  • Gbeta5
  • Inflammation
  • RGS protein
  • TNF-α
  • Trauma

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Hausmann, O. N., Hu, W. H., Keren-Raifman, T., Witherow, D. S., Wang, Q., Levay, K., ... Bethea, J. R. (2002). Spinal cord injury induces expression of RGS7 in microglia/macrophages in rats. European Journal of Neuroscience, 15(4), 602-612. https://doi.org/10.1046/j.1460-9568.2002.01916.x

Spinal cord injury induces expression of RGS7 in microglia/macrophages in rats. / Hausmann, Oliver N.; Hu, Wen Hui; Keren-Raifman, Tal; Witherow, D. Scott; Wang, Qiang; Levay, Konstantin; Frydel, Beata; Slepak, Vladlen Z; Bethea, John R.

In: European Journal of Neuroscience, Vol. 15, No. 4, 31.12.2002, p. 602-612.

Research output: Contribution to journalArticle

Hausmann, ON, Hu, WH, Keren-Raifman, T, Witherow, DS, Wang, Q, Levay, K, Frydel, B, Slepak, VZ & Bethea, JR 2002, 'Spinal cord injury induces expression of RGS7 in microglia/macrophages in rats', European Journal of Neuroscience, vol. 15, no. 4, pp. 602-612. https://doi.org/10.1046/j.1460-9568.2002.01916.x
Hausmann, Oliver N. ; Hu, Wen Hui ; Keren-Raifman, Tal ; Witherow, D. Scott ; Wang, Qiang ; Levay, Konstantin ; Frydel, Beata ; Slepak, Vladlen Z ; Bethea, John R. / Spinal cord injury induces expression of RGS7 in microglia/macrophages in rats. In: European Journal of Neuroscience. 2002 ; Vol. 15, No. 4. pp. 602-612.
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