Abstract
Transplantation of chromaffin cells into the lumbar subarachnoid space has been found to produce analgesia, most conspicuously against chronic neuropathic pain. To ascertain the neurophysiological mechanism, we recorded electrical activity from wide-dynamic-range dorsal horn neurons in vivo, measuring the short-lasting homosynaptic facilitatory effect known as windup, which is induced by repetitive C-fiber input. Rats were given adrenal medulla allografts, or, as controls, striated-muscle allografts. The adrenal-transplanted rats showed analgesia 3-4 wk after transplantation, measured as a reduction in flinching reflexes 30-55 min after subcutaneous formalin injection. Recordings were made under halothane anesthesia, 3-7 days following the behavioral testing. The average C-fiber response and subsequent afterdischarge were facilitated severalfold in control rats by 1-Hz cutaneous electrical stimulation. Such facilitation was essentially absent in adrenal-transplanted animals and also in the A-fiber response of both preparations. Extirpation of transplanted tissue several hours prior to recording did not significantly affect this difference. In conclusion, the adrenal transplants block short-term spinal nociceptive facilitation, probably by stimulating some persistent cellular process that may be an important determinant, but not the only one, of their analgesic effect.
| Original language | English |
|---|---|
| Pages (from-to) | 1788-1792 |
| Number of pages | 5 |
| Journal | Journal of Neurophysiology |
| Volume | 85 |
| Issue number | 4 |
| State | Published - Apr 19 2001 |
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ASJC Scopus subject areas
- Physiology
- Neuroscience(all)
Cite this
Spinal allografts of adrenal medulla block nociceptive facilitation in the dorsal horn. / Hentall, Ian; Noga, Brian R; Sagen, Jacqueline.
In: Journal of Neurophysiology, Vol. 85, No. 4, 19.04.2001, p. 1788-1792.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Spinal allografts of adrenal medulla block nociceptive facilitation in the dorsal horn
AU - Hentall, Ian
AU - Noga, Brian R
AU - Sagen, Jacqueline
PY - 2001/4/19
Y1 - 2001/4/19
N2 - Transplantation of chromaffin cells into the lumbar subarachnoid space has been found to produce analgesia, most conspicuously against chronic neuropathic pain. To ascertain the neurophysiological mechanism, we recorded electrical activity from wide-dynamic-range dorsal horn neurons in vivo, measuring the short-lasting homosynaptic facilitatory effect known as windup, which is induced by repetitive C-fiber input. Rats were given adrenal medulla allografts, or, as controls, striated-muscle allografts. The adrenal-transplanted rats showed analgesia 3-4 wk after transplantation, measured as a reduction in flinching reflexes 30-55 min after subcutaneous formalin injection. Recordings were made under halothane anesthesia, 3-7 days following the behavioral testing. The average C-fiber response and subsequent afterdischarge were facilitated severalfold in control rats by 1-Hz cutaneous electrical stimulation. Such facilitation was essentially absent in adrenal-transplanted animals and also in the A-fiber response of both preparations. Extirpation of transplanted tissue several hours prior to recording did not significantly affect this difference. In conclusion, the adrenal transplants block short-term spinal nociceptive facilitation, probably by stimulating some persistent cellular process that may be an important determinant, but not the only one, of their analgesic effect.
AB - Transplantation of chromaffin cells into the lumbar subarachnoid space has been found to produce analgesia, most conspicuously against chronic neuropathic pain. To ascertain the neurophysiological mechanism, we recorded electrical activity from wide-dynamic-range dorsal horn neurons in vivo, measuring the short-lasting homosynaptic facilitatory effect known as windup, which is induced by repetitive C-fiber input. Rats were given adrenal medulla allografts, or, as controls, striated-muscle allografts. The adrenal-transplanted rats showed analgesia 3-4 wk after transplantation, measured as a reduction in flinching reflexes 30-55 min after subcutaneous formalin injection. Recordings were made under halothane anesthesia, 3-7 days following the behavioral testing. The average C-fiber response and subsequent afterdischarge were facilitated severalfold in control rats by 1-Hz cutaneous electrical stimulation. Such facilitation was essentially absent in adrenal-transplanted animals and also in the A-fiber response of both preparations. Extirpation of transplanted tissue several hours prior to recording did not significantly affect this difference. In conclusion, the adrenal transplants block short-term spinal nociceptive facilitation, probably by stimulating some persistent cellular process that may be an important determinant, but not the only one, of their analgesic effect.
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UR - http://www.scopus.com/inward/citedby.url?scp=0035070148&partnerID=8YFLogxK
M3 - Article
C2 - 11287501
AN - SCOPUS:0035070148
VL - 85
SP - 1788
EP - 1792
JO - Journal of Neurophysiology
JF - Journal of Neurophysiology
SN - 0022-3077
IS - 4
ER -