Sphingolipids and ceramides of mouse aqueous humor: Comparative profiles from normotensive and hypertensive DBA/2J mice

Genea Edwards, Katyayini Aribindi, Yenifer Guerra, Sanjoy K. Bhattacharya

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Purpose To identify the sphingolipid and ceramide species and their quantitative differences between normotensive and hypertensive intraocular pressure states in DBA/2J mouse aqueous humor (AH).

Methods Normotensive and hypertensive AH was sampled from mice by paracentesis. Lipid extraction was performed using modifications of the Bligh and Dyer method. Protein concentration was estimated using the Bradford colorimetric assay. Sphingolipids and ceramides were identified and subjected to ratiometric quantification using appropriate class specific lipid standards on a TSQ Quantum Access Max triple quadrupole mass spectrometer.

Results The comparative profiles of normotensive and hypertensive DBA/2J mouse AH showed several species of sphingomyelin, sphingoid base, sphingoid base-1-phosphate (S1P) and ceramides common between them. A number of unique lipids in each of the above lipid classes were also identified in normotensive AH that were absent in hypertensive AH and vice versa.

Conclusion A number of sphingolipid and ceramide species were found to be uniquely present in normotensive, but absent in hypertensive AH and vice versa. Further pursuit of these findings is likely to contribute towards expanding our understanding of the molecular changes associated with increased intraocular pressure (IOP) and glaucoma.

Original languageEnglish (US)
Pages (from-to)99-109
Number of pages11
JournalBiochimie
Volume105
DOIs
StatePublished - Oct 2014

Keywords

  • Aqueous humor
  • Ceramides
  • Hypertensive
  • Mass spectrometry
  • Normotensive
  • Sphingolipids

ASJC Scopus subject areas

  • Biochemistry

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