Sphingolipids and ceramides in human aqueous humor

Ayman J. Aljohani, Gustavo C. Munguba, Yenifer Guerra, Richard K Lee, Sanjoy K Bhattacharya

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Purpose: To determine the differential profiles of sphingomyelin, sphingoid base, sphingoid base-1-phosphate and ceramide lipid species and their quantitative differences between control and glaucomatous aqueous humor (AQH) derived from human donors. Methods: AQH from control and primary open-angle glaucoma donors was collected and subjected to lipid extraction using suitable modifications of the Bligh and Dyer method. Proteins were estimated using Bradford's method. Lipids were identified and ratiometrically quantified in a two-step process using precursor ion scan or neutral loss scan (NLS) with appropriate class-specific lipid standards on a TSQ Quantum Access Max mass spectrometer following established procedures. Primary human trabecular meshwork cells and video microscopic imaging were used to assess changes in cell shape and motility upon exposure to 20 pmol of Cer(d18:0/18:1(9Z)) in 10% dimethyl sulfoxide (vehicle). Results: We identified several species of sphingomyelin, sphingoid base, sphingoid base-1-phosphate, and ceramides that were common between control and glaucomatous AQH. Some unique lipid species in these classes were also identified in controls but not in glaucoma and vice versa. We found exposure to 20 pmol of Cer(d18:0/18:1(9Z)) resulted in changes in the trabecular meshwork cell shape and observed motility changes compared to vehicle-only control. Conclusions: Most lipids belonging to the sphingomyelin, sphingoid base, sphingoid base-1-phosphate, and ceramide species were common between control and primary open-angle glaucoma donors. However, some sphingolipids and ceramides were found to be uniquely present in control but absent in the glaucomatous AQH and vice versa. Identification of unique lipid species present or absent in the pathophysiological context may contribute further insight into glaucoma pathology.

Original languageEnglish
Pages (from-to)1966-1984
Number of pages19
JournalMolecular Vision
Volume19
StatePublished - Sep 19 2013

Fingerprint

Sphingolipids
Aqueous Humor
Ceramides
Lipids
Sphingomyelins
Trabecular Meshwork
Cell Shape
Glaucoma
Dimethyl Sulfoxide
Cell Movement
Ions
Pathology
ceramide 1-phosphate

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Aljohani, A. J., Munguba, G. C., Guerra, Y., Lee, R. K., & Bhattacharya, S. K. (2013). Sphingolipids and ceramides in human aqueous humor. Molecular Vision, 19, 1966-1984.

Sphingolipids and ceramides in human aqueous humor. / Aljohani, Ayman J.; Munguba, Gustavo C.; Guerra, Yenifer; Lee, Richard K; Bhattacharya, Sanjoy K.

In: Molecular Vision, Vol. 19, 19.09.2013, p. 1966-1984.

Research output: Contribution to journalArticle

Aljohani, AJ, Munguba, GC, Guerra, Y, Lee, RK & Bhattacharya, SK 2013, 'Sphingolipids and ceramides in human aqueous humor', Molecular Vision, vol. 19, pp. 1966-1984.
Aljohani AJ, Munguba GC, Guerra Y, Lee RK, Bhattacharya SK. Sphingolipids and ceramides in human aqueous humor. Molecular Vision. 2013 Sep 19;19:1966-1984.
Aljohani, Ayman J. ; Munguba, Gustavo C. ; Guerra, Yenifer ; Lee, Richard K ; Bhattacharya, Sanjoy K. / Sphingolipids and ceramides in human aqueous humor. In: Molecular Vision. 2013 ; Vol. 19. pp. 1966-1984.
@article{a1463c8016dc4199853db96d3887c7dc,
title = "Sphingolipids and ceramides in human aqueous humor",
abstract = "Purpose: To determine the differential profiles of sphingomyelin, sphingoid base, sphingoid base-1-phosphate and ceramide lipid species and their quantitative differences between control and glaucomatous aqueous humor (AQH) derived from human donors. Methods: AQH from control and primary open-angle glaucoma donors was collected and subjected to lipid extraction using suitable modifications of the Bligh and Dyer method. Proteins were estimated using Bradford's method. Lipids were identified and ratiometrically quantified in a two-step process using precursor ion scan or neutral loss scan (NLS) with appropriate class-specific lipid standards on a TSQ Quantum Access Max mass spectrometer following established procedures. Primary human trabecular meshwork cells and video microscopic imaging were used to assess changes in cell shape and motility upon exposure to 20 pmol of Cer(d18:0/18:1(9Z)) in 10{\%} dimethyl sulfoxide (vehicle). Results: We identified several species of sphingomyelin, sphingoid base, sphingoid base-1-phosphate, and ceramides that were common between control and glaucomatous AQH. Some unique lipid species in these classes were also identified in controls but not in glaucoma and vice versa. We found exposure to 20 pmol of Cer(d18:0/18:1(9Z)) resulted in changes in the trabecular meshwork cell shape and observed motility changes compared to vehicle-only control. Conclusions: Most lipids belonging to the sphingomyelin, sphingoid base, sphingoid base-1-phosphate, and ceramide species were common between control and primary open-angle glaucoma donors. However, some sphingolipids and ceramides were found to be uniquely present in control but absent in the glaucomatous AQH and vice versa. Identification of unique lipid species present or absent in the pathophysiological context may contribute further insight into glaucoma pathology.",
author = "Aljohani, {Ayman J.} and Munguba, {Gustavo C.} and Yenifer Guerra and Lee, {Richard K} and Bhattacharya, {Sanjoy K}",
year = "2013",
month = "9",
day = "19",
language = "English",
volume = "19",
pages = "1966--1984",
journal = "Molecular Vision",
issn = "1090-0535",

}

TY - JOUR

T1 - Sphingolipids and ceramides in human aqueous humor

AU - Aljohani, Ayman J.

AU - Munguba, Gustavo C.

AU - Guerra, Yenifer

AU - Lee, Richard K

AU - Bhattacharya, Sanjoy K

PY - 2013/9/19

Y1 - 2013/9/19

N2 - Purpose: To determine the differential profiles of sphingomyelin, sphingoid base, sphingoid base-1-phosphate and ceramide lipid species and their quantitative differences between control and glaucomatous aqueous humor (AQH) derived from human donors. Methods: AQH from control and primary open-angle glaucoma donors was collected and subjected to lipid extraction using suitable modifications of the Bligh and Dyer method. Proteins were estimated using Bradford's method. Lipids were identified and ratiometrically quantified in a two-step process using precursor ion scan or neutral loss scan (NLS) with appropriate class-specific lipid standards on a TSQ Quantum Access Max mass spectrometer following established procedures. Primary human trabecular meshwork cells and video microscopic imaging were used to assess changes in cell shape and motility upon exposure to 20 pmol of Cer(d18:0/18:1(9Z)) in 10% dimethyl sulfoxide (vehicle). Results: We identified several species of sphingomyelin, sphingoid base, sphingoid base-1-phosphate, and ceramides that were common between control and glaucomatous AQH. Some unique lipid species in these classes were also identified in controls but not in glaucoma and vice versa. We found exposure to 20 pmol of Cer(d18:0/18:1(9Z)) resulted in changes in the trabecular meshwork cell shape and observed motility changes compared to vehicle-only control. Conclusions: Most lipids belonging to the sphingomyelin, sphingoid base, sphingoid base-1-phosphate, and ceramide species were common between control and primary open-angle glaucoma donors. However, some sphingolipids and ceramides were found to be uniquely present in control but absent in the glaucomatous AQH and vice versa. Identification of unique lipid species present or absent in the pathophysiological context may contribute further insight into glaucoma pathology.

AB - Purpose: To determine the differential profiles of sphingomyelin, sphingoid base, sphingoid base-1-phosphate and ceramide lipid species and their quantitative differences between control and glaucomatous aqueous humor (AQH) derived from human donors. Methods: AQH from control and primary open-angle glaucoma donors was collected and subjected to lipid extraction using suitable modifications of the Bligh and Dyer method. Proteins were estimated using Bradford's method. Lipids were identified and ratiometrically quantified in a two-step process using precursor ion scan or neutral loss scan (NLS) with appropriate class-specific lipid standards on a TSQ Quantum Access Max mass spectrometer following established procedures. Primary human trabecular meshwork cells and video microscopic imaging were used to assess changes in cell shape and motility upon exposure to 20 pmol of Cer(d18:0/18:1(9Z)) in 10% dimethyl sulfoxide (vehicle). Results: We identified several species of sphingomyelin, sphingoid base, sphingoid base-1-phosphate, and ceramides that were common between control and glaucomatous AQH. Some unique lipid species in these classes were also identified in controls but not in glaucoma and vice versa. We found exposure to 20 pmol of Cer(d18:0/18:1(9Z)) resulted in changes in the trabecular meshwork cell shape and observed motility changes compared to vehicle-only control. Conclusions: Most lipids belonging to the sphingomyelin, sphingoid base, sphingoid base-1-phosphate, and ceramide species were common between control and primary open-angle glaucoma donors. However, some sphingolipids and ceramides were found to be uniquely present in control but absent in the glaucomatous AQH and vice versa. Identification of unique lipid species present or absent in the pathophysiological context may contribute further insight into glaucoma pathology.

UR - http://www.scopus.com/inward/record.url?scp=84884359540&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884359540&partnerID=8YFLogxK

M3 - Article

C2 - 24068864

AN - SCOPUS:84884359540

VL - 19

SP - 1966

EP - 1984

JO - Molecular Vision

JF - Molecular Vision

SN - 1090-0535

ER -