Sperm of the Shrimp Sicyonia ingentis Undergo a Bi‐Phasic Capacitation Accompanied by Morphological Changes

Athula H. Wikramanayake, Kevin R. Uhlinger, Fred J. Griffin, Wallis H. Clark

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Sperm removed from seminal receptacles of female Sicyonia ingentis can be induced to undergo a bi‐phasic acrosome reaction (AR), acrosomal exocytosis followed by filament formation, using egg water (EW). Sperm removed from males will not undergo any phase of the AR when incubated with EW, indicating that these sperm undergo a capacitation process after insemination. Freshly molted females (functional virgins) were placed in aquaria with males and monitored for copulation. Mated females were isolated and allowed to carry sperm for specific periods of time. At these time points, sperm were removed and assayed for the ability to undergo the AR using EW. The results indicate that sperm are competent to undergo acrosomal exocytosis after approximately 25 hr, while competency to form acrosomal filaments is not achieved until around 145 hr post‐insemination. Morphological examination of sperm removed from males and sperm removed from females revealed dramatic differences. Microscopic evidence indicates that some of the morphological changes seen during capacitation are necessary for the successful completion of the AR.

Original languageEnglish (US)
Pages (from-to)347-355
Number of pages9
JournalDevelopment, Growth & Differentiation
Volume34
Issue number3
DOIs
StatePublished - Jun 1992
Externally publishedYes

Keywords

  • (shrimp
  • acrosome reaction
  • morphological changes
  • sperm capacitation

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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