Spectrophotometric determination of lantadene A, 22β-angeloxy-3-oxoolean-12-en-28-oic acid

O. P. Sharma, H. P.S. Makkar, R. K. Dawra

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The formation of a blue chromogen between sodium borohydride-treated lantadene A (22β-angeloyloxy-3-oxoolean-12-en-28-oic acid) and acetic anhydride-sulfuric acid (9:1) formed the basis of a spectrophotometric method for its quantitation. The chromogen had a broad absorption maximum (λmax) at 630-645 nm. The optimum amount of sodium borohydride for lantadene A reduction was 1 mg/mg lantadene A in methanolic solution. The chromogen was stable for 5, 7, and 26 min after reaction at 25, 18, and 0°C, respectively. The method is convenient, sensitive, and reproducible. The amount of lantadene A in the leaves of Lantana camara collected in the month of May quantitated by the present method was found to be 13.6 mg/g dry weight of the leaves.

Original languageEnglish (US)
Pages (from-to)474-477
Number of pages4
JournalAnalytical Biochemistry
Volume128
Issue number2
DOIs
StatePublished - Feb 1 1983
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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