Abstract
The purpose of this study is to demonstrate the application of ultrahigh-resolution Spectral Domain Optical Coherence Tomography (SD-OCT) for non contact in vivo imaging of the retina of small animals and quantitative retinal information extraction using 3D segmentation of the OCT images. An ultrahigh-resolution SD-OCT system was specifically designed for in vivo retinal imaging of small animal. En face fondus image was constructed from the measured OCT data, which enables precise registration of the OCT images on the fondus. 3D segmentation algorithms were developed for the calculation of retinal thickness map. High quality OCT images of the retina of mice (B6/SJLF2 for normal retina, Rho-/- for photoreceptor degeneration and LH BETATAG for retinoblastoma) and rats (Wistar for normal retina) were acquired, where all the retinal layers can be clearly recognized. The calculated retinal thickness map makes successful quantitative comparison of the retinal thickness distribution between normal and degenerative mouse retina. The capabilities of the OCT system provide a valuable tool for longitudinal studies of small animal models of ocular diseases.
| Original language | English |
|---|---|
| Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume | 6429 |
| DOIs | |
| State | Published - May 22 2007 |
| Event | Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine XI - San Jose, CA, United States Duration: Jan 22 2007 → Jan 24 2007 |
Other
| Other | Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine XI |
|---|---|
| Country | United States |
| City | San Jose, CA |
| Period | 1/22/07 → 1/24/07 |
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Keywords
- Imaging
- Optical coherence tomography
- Retina
ASJC Scopus subject areas
- Engineering(all)
Cite this
Spectral domain optical coherence tomography for in-vivo three dimensional retinal imaging of small animals. / Ruggeri, Marco; Wehbe, Hassan; Jiao, Shuliang; Gregori, Giovanni; Jockovich, Maria E.; Hackam, Abigail S; Duan, Yuanli; Puliafito, Carmen A.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6429 2007. 64290S.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Spectral domain optical coherence tomography for in-vivo three dimensional retinal imaging of small animals
AU - Ruggeri, Marco
AU - Wehbe, Hassan
AU - Jiao, Shuliang
AU - Gregori, Giovanni
AU - Jockovich, Maria E.
AU - Hackam, Abigail S
AU - Duan, Yuanli
AU - Puliafito, Carmen A.
PY - 2007/5/22
Y1 - 2007/5/22
N2 - The purpose of this study is to demonstrate the application of ultrahigh-resolution Spectral Domain Optical Coherence Tomography (SD-OCT) for non contact in vivo imaging of the retina of small animals and quantitative retinal information extraction using 3D segmentation of the OCT images. An ultrahigh-resolution SD-OCT system was specifically designed for in vivo retinal imaging of small animal. En face fondus image was constructed from the measured OCT data, which enables precise registration of the OCT images on the fondus. 3D segmentation algorithms were developed for the calculation of retinal thickness map. High quality OCT images of the retina of mice (B6/SJLF2 for normal retina, Rho-/- for photoreceptor degeneration and LH BETATAG for retinoblastoma) and rats (Wistar for normal retina) were acquired, where all the retinal layers can be clearly recognized. The calculated retinal thickness map makes successful quantitative comparison of the retinal thickness distribution between normal and degenerative mouse retina. The capabilities of the OCT system provide a valuable tool for longitudinal studies of small animal models of ocular diseases.
AB - The purpose of this study is to demonstrate the application of ultrahigh-resolution Spectral Domain Optical Coherence Tomography (SD-OCT) for non contact in vivo imaging of the retina of small animals and quantitative retinal information extraction using 3D segmentation of the OCT images. An ultrahigh-resolution SD-OCT system was specifically designed for in vivo retinal imaging of small animal. En face fondus image was constructed from the measured OCT data, which enables precise registration of the OCT images on the fondus. 3D segmentation algorithms were developed for the calculation of retinal thickness map. High quality OCT images of the retina of mice (B6/SJLF2 for normal retina, Rho-/- for photoreceptor degeneration and LH BETATAG for retinoblastoma) and rats (Wistar for normal retina) were acquired, where all the retinal layers can be clearly recognized. The calculated retinal thickness map makes successful quantitative comparison of the retinal thickness distribution between normal and degenerative mouse retina. The capabilities of the OCT system provide a valuable tool for longitudinal studies of small animal models of ocular diseases.
KW - Imaging
KW - Optical coherence tomography
KW - Retina
UR - http://www.scopus.com/inward/record.url?scp=34248530200&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34248530200&partnerID=8YFLogxK
U2 - 10.1117/12.700437
DO - 10.1117/12.700437
M3 - Conference contribution
AN - SCOPUS:34248530200
SN - 0819465429
SN - 9780819465429
VL - 6429
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
ER -