Spectral domain optical coherence tomography for in-vivo three dimensional retinal imaging of small animals

Marco Ruggeri, Hassan Wehbe, Shuliang Jiao, Giovanni Gregori, Maria E. Jockovich, Abigail Hackam, Yuanli Duan, Carmen A. Puliafito

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The purpose of this study is to demonstrate the application of ultrahigh-resolution Spectral Domain Optical Coherence Tomography (SD-OCT) for non contact in vivo imaging of the retina of small animals and quantitative retinal information extraction using 3D segmentation of the OCT images. An ultrahigh-resolution SD-OCT system was specifically designed for in vivo retinal imaging of small animal. En face fondus image was constructed from the measured OCT data, which enables precise registration of the OCT images on the fondus. 3D segmentation algorithms were developed for the calculation of retinal thickness map. High quality OCT images of the retina of mice (B6/SJLF2 for normal retina, Rho-/- for photoreceptor degeneration and LH BETATAG for retinoblastoma) and rats (Wistar for normal retina) were acquired, where all the retinal layers can be clearly recognized. The calculated retinal thickness map makes successful quantitative comparison of the retinal thickness distribution between normal and degenerative mouse retina. The capabilities of the OCT system provide a valuable tool for longitudinal studies of small animal models of ocular diseases.

Original languageEnglish (US)
Title of host publicationCoherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine XI
DOIs
StatePublished - May 22 2007
EventCoherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine XI - San Jose, CA, United States
Duration: Jan 22 2007Jan 24 2007

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6429
ISSN (Print)1605-7422

Other

OtherCoherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine XI
CountryUnited States
CitySan Jose, CA
Period1/22/071/24/07

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Keywords

  • Imaging
  • Optical coherence tomography
  • Retina

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Ruggeri, M., Wehbe, H., Jiao, S., Gregori, G., Jockovich, M. E., Hackam, A., Duan, Y., & Puliafito, C. A. (2007). Spectral domain optical coherence tomography for in-vivo three dimensional retinal imaging of small animals. In Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine XI [64290S] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6429). https://doi.org/10.1117/12.700437