Specification of Dendritogenesis Site in Drosophila aCC Motoneuron by Membrane Enrichment of Pak1 through Dscam1

Daichi Kamiyama, Ryan McGorty, Rie Kamiyama, Michael D. Kim, Akira Chiba, Bo Huang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Precise positioning of dendritic branches is a critical step in the establishment of neuronal circuitry. However, there is limited knowledge on how environmental cues translate into dendrite initiation or branching at a specific position. Here, through a combination of mutation, RNAi, and imaging experiments, we found that a Dscam-Dock-Pak1 hierarchical interaction defines the stereotypical dendrite growth site in the Drosophila aCC motoneuron. This interaction localizes the Cdc42 effector Pak1 to the plasma membrane at the dendrite initiation site before the activation of Cdc42. Ectopic expression of membrane-anchored Pak1 overrides this spatial specification of dendritogenesis, confirming its function in guiding Cdc42 signaling. We further discovered that Dscam1 localization in aCC occurs through an inter-neuronal contact that involves Dscam1 in the partner MP1 neuron. These findings elucidate a mechanism by which Dscam1 controls neuronal morphogenesis through spatial regulation of Cdc42 signaling and, subsequently, cytoskeletal remodeling. Proper neuronal wiring requires precise positioning of dendritic branches. Kamiyama et al. show that a Dscam1-Dock-Pak1 hierarchical interaction defines the site of dendritogenesis in the aCC motoneuron in Drosophila, in part via Dscam1-dependent inter-neuronal interactions that specify Dscam1 localization in aCC.

Original languageEnglish (US)
Article number3397
Pages (from-to)93-106
Number of pages14
JournalDevelopmental Cell
Volume35
Issue number1
DOIs
StatePublished - Oct 12 2015

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Docks
Motor Neurons
Dendrites
Drosophila
Membranes
Specifications
Electric wiring
Cell membranes
Neurons
Chemical activation
RNA Interference
Morphogenesis
Imaging techniques
Cues
Cell Membrane
Mutation
Experiments
Growth

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Specification of Dendritogenesis Site in Drosophila aCC Motoneuron by Membrane Enrichment of Pak1 through Dscam1. / Kamiyama, Daichi; McGorty, Ryan; Kamiyama, Rie; Kim, Michael D.; Chiba, Akira; Huang, Bo.

In: Developmental Cell, Vol. 35, No. 1, 3397, 12.10.2015, p. 93-106.

Research output: Contribution to journalArticle

Kamiyama, Daichi ; McGorty, Ryan ; Kamiyama, Rie ; Kim, Michael D. ; Chiba, Akira ; Huang, Bo. / Specification of Dendritogenesis Site in Drosophila aCC Motoneuron by Membrane Enrichment of Pak1 through Dscam1. In: Developmental Cell. 2015 ; Vol. 35, No. 1. pp. 93-106.
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