Specification of actin filament function and molecular composition by tropomyosin isoforms

Nicole S. Bryce, Galina Schevzov, Vicki Ferguson, Justin M. Percival, Jim J.C. Lin, Fumio Matsumura, James R. Bamburg, Peter L. Jeffrey, Edna C. Hardeman, Peter Gunning, Ron P. Weinberger

Research output: Contribution to journalArticlepeer-review

176 Scopus citations

Abstract

The specific functions of greater than 40 vertebrate nonmuscle tropomyosins (Tms) are poorly understood. In this article we have tested the ability of two Tm isoforms, TmBr3 and the human homologue of Tm5 (hTM5NM1), to regulate actin filament function. We found that these Tms can differentially alter actin filament organization, cell size, and shape, hTm5NM1 was able to recruit myosin II into stress fibers, which resulted in decreased lamellipodia and cellular migration. In contrast, TmBr3 transfection induced lamellipodial formation, increased cellular migration, and reduced stress fibers. Based on coimmunoprecipitation and colocalization studies, TmBr3 appeared to be associated with actin-depolymerizing factor/cofilin (ADF)-bound actin filaments. Additionally, the Tms can specifically regulate the incorporation of other Tms into actin filaments, suggesting that selective dimerization may also be involved in the control of actin filament organization. We conclude that Tm isoforms can be used to specify the functional properties and molecular composition of actin filaments and that spatial segregation of isoforms may lead to localized specialization of actin filament function.

Original languageEnglish (US)
Pages (from-to)1002-1016
Number of pages15
JournalMolecular biology of the cell
Volume14
Issue number3
DOIs
StatePublished - Mar 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Specification of actin filament function and molecular composition by tropomyosin isoforms'. Together they form a unique fingerprint.

Cite this