Specific cytotoxicity of 16 alpha-[125I]iodoestradiol for estrogen receptor-containing breast cancer cells.

D. A. Bronzert, R. B. Hochberg, Marc E Lippman

Research output: Contribution to journalArticle

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Abstract

We attempted to induce specific killing of estrogen receptor-containing breast cancer cells using estradiol coupled to a high specific activity gamma emitting isotope of iodine. MCF-7 human breast cancer cells were incubated with 16 alpha-[125I]iodoestradiol alone ([125I]E2), or with 16 alpha-[125I]iodoestradiol plus 100-fold excess 17 beta-estradiol (E2), and then viably frozen. After 8 weeks, the [125I]E2 exposed cells and the [125 I]E2 plus E2 exposed cells were 10% and 77% of controls, respectively. When the breast cancer cell line MDA-MB-231 which does not contain estrogen receptors was used, the rate of cell death was similar to the competed MCF-7 cells. When specific cytotoxicity was compared using a cloning technique, nearly a 5 log reduction in surviving cell fraction was seen with [125I]E2, as compared to identical cells treated with [125I]E2 plus competitor. This technique shows promise for selecting a population of cells with defects in their estrogen receptor and in studying subcellular hormone interactions.

Original languageEnglish
Pages (from-to)2177-2182
Number of pages6
JournalEndocrinology
Volume110
Issue number6
StatePublished - Jun 1 1982
Externally publishedYes

Fingerprint

Estrogen Receptors
Breast Neoplasms
Iodine Isotopes
Estradiol
MCF-7 Cells
16 alpha-iodoestradiol
Organism Cloning
Cell Death
Hormones
Cell Line
Population

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Bronzert, D. A., Hochberg, R. B., & Lippman, M. E. (1982). Specific cytotoxicity of 16 alpha-[125I]iodoestradiol for estrogen receptor-containing breast cancer cells. Endocrinology, 110(6), 2177-2182.

Specific cytotoxicity of 16 alpha-[125I]iodoestradiol for estrogen receptor-containing breast cancer cells. / Bronzert, D. A.; Hochberg, R. B.; Lippman, Marc E.

In: Endocrinology, Vol. 110, No. 6, 01.06.1982, p. 2177-2182.

Research output: Contribution to journalArticle

Bronzert, DA, Hochberg, RB & Lippman, ME 1982, 'Specific cytotoxicity of 16 alpha-[125I]iodoestradiol for estrogen receptor-containing breast cancer cells.', Endocrinology, vol. 110, no. 6, pp. 2177-2182.
Bronzert, D. A. ; Hochberg, R. B. ; Lippman, Marc E. / Specific cytotoxicity of 16 alpha-[125I]iodoestradiol for estrogen receptor-containing breast cancer cells. In: Endocrinology. 1982 ; Vol. 110, No. 6. pp. 2177-2182.
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