Specific cytotoxicity of 16 alpha-[125I]iodoestradiol for estrogen receptor-containing breast cancer cells.

D. A. Bronzert, R. B. Hochberg, M. E. Lippman

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

We attempted to induce specific killing of estrogen receptor-containing breast cancer cells using estradiol coupled to a high specific activity gamma emitting isotope of iodine. MCF-7 human breast cancer cells were incubated with 16 alpha-[125I]iodoestradiol alone ([125I]E2), or with 16 alpha-[125I]iodoestradiol plus 100-fold excess 17 beta-estradiol (E2), and then viably frozen. After 8 weeks, the [125I]E2 exposed cells and the [125 I]E2 plus E2 exposed cells were 10% and 77% of controls, respectively. When the breast cancer cell line MDA-MB-231 which does not contain estrogen receptors was used, the rate of cell death was similar to the competed MCF-7 cells. When specific cytotoxicity was compared using a cloning technique, nearly a 5 log reduction in surviving cell fraction was seen with [125I]E2, as compared to identical cells treated with [125I]E2 plus competitor. This technique shows promise for selecting a population of cells with defects in their estrogen receptor and in studying subcellular hormone interactions.

Original languageEnglish (US)
Pages (from-to)2177-2182
Number of pages6
JournalEndocrinology
Volume110
Issue number6
StatePublished - Jun 1 1982

ASJC Scopus subject areas

  • Endocrinology

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    Bronzert, D. A., Hochberg, R. B., & Lippman, M. E. (1982). Specific cytotoxicity of 16 alpha-[125I]iodoestradiol for estrogen receptor-containing breast cancer cells. Endocrinology, 110(6), 2177-2182.