Specific antibodies directed against toxins of Ptychodiscus brevis (Florida's red tide dinoflagellate)

Daniel G. Baden, Thomas J. Mende, Jean Walling, Duane R. Schultz

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Specific antibodies directed against Ptychodiscus brevis 'brevetoxins' have been produced in a goat. The haptenic toxin T34 was chemically reduced to toxin T17, covalently-linked to succinic acid via anhydride coupling, and coupled to bovine serum albumin using standard carbodiimide condensation procedures. The hapten coupling efficiency ranged from 10.4 to 13.5 moles of toxin bound per mole of protein. Antibody titers were directly related to the frequency of immunization, and weekly intervals appeared optimum for maintaining adequate titers. [3H]Brevetoxin T17 is displaced in a competitive manner from the antibody - antigen complexes by unlabeled toxin, but the antibodies do not distinguish between T17 and T34. The sensitivity achieved, using purified brevetoxins as competitive inhibitors of [3H]T17 binding, was 600 picograms. The assay linearity ranged from 1.5 to 48 ng.

Original languageEnglish
Pages (from-to)783-789
Number of pages7
JournalToxicon
Volume22
Issue number5
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

Fingerprint

Harmful Algal Bloom
Dinoflagellida
Tides
Antibodies
Immunization
Carbodiimides
Anhydrides
Haptens
Succinic Acid
Bovine Serum Albumin
Antigen-Antibody Complex
Goats
Condensation
Assays
Proteins
brevetoxin T17
brevetoxin

ASJC Scopus subject areas

  • Toxicology

Cite this

Specific antibodies directed against toxins of Ptychodiscus brevis (Florida's red tide dinoflagellate). / Baden, Daniel G.; Mende, Thomas J.; Walling, Jean; Schultz, Duane R.

In: Toxicon, Vol. 22, No. 5, 01.01.1984, p. 783-789.

Research output: Contribution to journalArticle

Baden, Daniel G. ; Mende, Thomas J. ; Walling, Jean ; Schultz, Duane R. / Specific antibodies directed against toxins of Ptychodiscus brevis (Florida's red tide dinoflagellate). In: Toxicon. 1984 ; Vol. 22, No. 5. pp. 783-789.
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