Specific antibodies directed against toxins of Ptychodiscus brevis (Florida's red tide dinoflagellate)

Daniel G. Baden; Thomas J. Mende; Jean Walling; Duane R. Schultz

doi:10.1016/0041-0101(84)90161-2

Specific antibodies directed against toxins of Ptychodiscus brevis (Florida's red tide dinoflagellate)

Daniel G. Baden, Thomas J. Mende, Jean Walling, Duane R. Schultz

Medicine

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Specific antibodies directed against Ptychodiscus brevis 'brevetoxins' have been produced in a goat. The haptenic toxin T34 was chemically reduced to toxin T17, covalently-linked to succinic acid via anhydride coupling, and coupled to bovine serum albumin using standard carbodiimide condensation procedures. The hapten coupling efficiency ranged from 10.4 to 13.5 moles of toxin bound per mole of protein. Antibody titers were directly related to the frequency of immunization, and weekly intervals appeared optimum for maintaining adequate titers. [³H]Brevetoxin T17 is displaced in a competitive manner from the antibody - antigen complexes by unlabeled toxin, but the antibodies do not distinguish between T17 and T34. The sensitivity achieved, using purified brevetoxins as competitive inhibitors of [³H]T17 binding, was 600 picograms. The assay linearity ranged from 1.5 to 48 ng.

Original language	English (US)
Pages (from-to)	783-789
Number of pages	7
Journal	Toxicon
Volume	22
Issue number	5
DOIs	https://doi.org/10.1016/0041-0101(84)90161-2
State	Published - 1984

ASJC Scopus subject areas

Toxicology

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title = "Specific antibodies directed against toxins of Ptychodiscus brevis (Florida's red tide dinoflagellate)",

abstract = "Specific antibodies directed against Ptychodiscus brevis 'brevetoxins' have been produced in a goat. The haptenic toxin T34 was chemically reduced to toxin T17, covalently-linked to succinic acid via anhydride coupling, and coupled to bovine serum albumin using standard carbodiimide condensation procedures. The hapten coupling efficiency ranged from 10.4 to 13.5 moles of toxin bound per mole of protein. Antibody titers were directly related to the frequency of immunization, and weekly intervals appeared optimum for maintaining adequate titers. [3H]Brevetoxin T17 is displaced in a competitive manner from the antibody - antigen complexes by unlabeled toxin, but the antibodies do not distinguish between T17 and T34. The sensitivity achieved, using purified brevetoxins as competitive inhibitors of [3H]T17 binding, was 600 picograms. The assay linearity ranged from 1.5 to 48 ng.",

author = "Baden, {Daniel G.} and Mende, {Thomas J.} and Jean Walling and Schultz, {Duane R.}",

year = "1984",

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T1 - Specific antibodies directed against toxins of Ptychodiscus brevis (Florida's red tide dinoflagellate)

AU - Baden, Daniel G.

AU - Mende, Thomas J.

AU - Walling, Jean

AU - Schultz, Duane R.

PY - 1984

Y1 - 1984

N2 - Specific antibodies directed against Ptychodiscus brevis 'brevetoxins' have been produced in a goat. The haptenic toxin T34 was chemically reduced to toxin T17, covalently-linked to succinic acid via anhydride coupling, and coupled to bovine serum albumin using standard carbodiimide condensation procedures. The hapten coupling efficiency ranged from 10.4 to 13.5 moles of toxin bound per mole of protein. Antibody titers were directly related to the frequency of immunization, and weekly intervals appeared optimum for maintaining adequate titers. [3H]Brevetoxin T17 is displaced in a competitive manner from the antibody - antigen complexes by unlabeled toxin, but the antibodies do not distinguish between T17 and T34. The sensitivity achieved, using purified brevetoxins as competitive inhibitors of [3H]T17 binding, was 600 picograms. The assay linearity ranged from 1.5 to 48 ng.

AB - Specific antibodies directed against Ptychodiscus brevis 'brevetoxins' have been produced in a goat. The haptenic toxin T34 was chemically reduced to toxin T17, covalently-linked to succinic acid via anhydride coupling, and coupled to bovine serum albumin using standard carbodiimide condensation procedures. The hapten coupling efficiency ranged from 10.4 to 13.5 moles of toxin bound per mole of protein. Antibody titers were directly related to the frequency of immunization, and weekly intervals appeared optimum for maintaining adequate titers. [3H]Brevetoxin T17 is displaced in a competitive manner from the antibody - antigen complexes by unlabeled toxin, but the antibodies do not distinguish between T17 and T34. The sensitivity achieved, using purified brevetoxins as competitive inhibitors of [3H]T17 binding, was 600 picograms. The assay linearity ranged from 1.5 to 48 ng.

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Specific antibodies directed against toxins of Ptychodiscus brevis (Florida's red tide dinoflagellate)

Abstract

ASJC Scopus subject areas

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