Spatiotemporal analysis of zebrafish hox gene regulation by Cdx4

Albert G. Hayward, Piyush Joshi, Isaac Skromne

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Cdx factors expressed in caudal regions of vertebrate embryos regulate hox patterning gene transcription. While loss of Cdx function is known to shift hox spatial expression domains posteriorly, the mechanism underlying the shift is not understood. We addressed this problem by analyzing the spatiotemporal expression profile of all 49 zebrafish hox genes in wild-type and Cdx4-deficient embryos. Results: Loss of Cdx4 had distinct effects on hox spatial expression in a paralogous group-dependent manner: in the head, group 4 expression was expanded posteriorly; in the trunk, group 5-10 expression was shifted posteriorly; and in the tail, group 11-13 genes were expressed in the tail bud but not in more differentiated tissues. In the trunk neural tissue, loss of Cdx4 severely delayed both transcriptional activation of hox genes during the initiation phase, and the anterior-ward expansion of hox expression domains during the establishment phase. In contrast, in the trunk mesoderm, loss of Cdx4 only delayed the hox initiation phase. Conclusions: These results indicate that Cdx4 differentially regulates the transcription of head, trunk and tail hox genes. In the trunk, Cdx4 conveys spatial positional information to axial tissues primarily by regulating the time of hox gene transcriptional activation during the initiation phase.

Original languageEnglish (US)
Pages (from-to)1564-1573
Number of pages10
JournalDevelopmental Dynamics
Volume244
Issue number12
DOIs
StatePublished - Dec 1 2015

Fingerprint

Spatio-Temporal Analysis
Homeobox Genes
Zebrafish
Transcriptional Activation
Tail
Embryonic Structures
Mesoderm
Vertebrates
Head
Genes

Keywords

  • Axial patterning
  • Spatial collinearity
  • Temporal collinearity

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Spatiotemporal analysis of zebrafish hox gene regulation by Cdx4. / Hayward, Albert G.; Joshi, Piyush; Skromne, Isaac.

In: Developmental Dynamics, Vol. 244, No. 12, 01.12.2015, p. 1564-1573.

Research output: Contribution to journalArticle

Hayward, AG, Joshi, P & Skromne, I 2015, 'Spatiotemporal analysis of zebrafish hox gene regulation by Cdx4', Developmental Dynamics, vol. 244, no. 12, pp. 1564-1573. https://doi.org/10.1002/dvdy.24343
Hayward, Albert G. ; Joshi, Piyush ; Skromne, Isaac. / Spatiotemporal analysis of zebrafish hox gene regulation by Cdx4. In: Developmental Dynamics. 2015 ; Vol. 244, No. 12. pp. 1564-1573.
@article{df255fc3c6b84eeb85b7ad70dbdc0c90,
title = "Spatiotemporal analysis of zebrafish hox gene regulation by Cdx4",
abstract = "Background: Cdx factors expressed in caudal regions of vertebrate embryos regulate hox patterning gene transcription. While loss of Cdx function is known to shift hox spatial expression domains posteriorly, the mechanism underlying the shift is not understood. We addressed this problem by analyzing the spatiotemporal expression profile of all 49 zebrafish hox genes in wild-type and Cdx4-deficient embryos. Results: Loss of Cdx4 had distinct effects on hox spatial expression in a paralogous group-dependent manner: in the head, group 4 expression was expanded posteriorly; in the trunk, group 5-10 expression was shifted posteriorly; and in the tail, group 11-13 genes were expressed in the tail bud but not in more differentiated tissues. In the trunk neural tissue, loss of Cdx4 severely delayed both transcriptional activation of hox genes during the initiation phase, and the anterior-ward expansion of hox expression domains during the establishment phase. In contrast, in the trunk mesoderm, loss of Cdx4 only delayed the hox initiation phase. Conclusions: These results indicate that Cdx4 differentially regulates the transcription of head, trunk and tail hox genes. In the trunk, Cdx4 conveys spatial positional information to axial tissues primarily by regulating the time of hox gene transcriptional activation during the initiation phase.",
keywords = "Axial patterning, Spatial collinearity, Temporal collinearity",
author = "Hayward, {Albert G.} and Piyush Joshi and Isaac Skromne",
year = "2015",
month = "12",
day = "1",
doi = "10.1002/dvdy.24343",
language = "English (US)",
volume = "244",
pages = "1564--1573",
journal = "Developmental Dynamics",
issn = "1058-8388",
publisher = "Wiley-Liss Inc.",
number = "12",

}

TY - JOUR

T1 - Spatiotemporal analysis of zebrafish hox gene regulation by Cdx4

AU - Hayward, Albert G.

AU - Joshi, Piyush

AU - Skromne, Isaac

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Background: Cdx factors expressed in caudal regions of vertebrate embryos regulate hox patterning gene transcription. While loss of Cdx function is known to shift hox spatial expression domains posteriorly, the mechanism underlying the shift is not understood. We addressed this problem by analyzing the spatiotemporal expression profile of all 49 zebrafish hox genes in wild-type and Cdx4-deficient embryos. Results: Loss of Cdx4 had distinct effects on hox spatial expression in a paralogous group-dependent manner: in the head, group 4 expression was expanded posteriorly; in the trunk, group 5-10 expression was shifted posteriorly; and in the tail, group 11-13 genes were expressed in the tail bud but not in more differentiated tissues. In the trunk neural tissue, loss of Cdx4 severely delayed both transcriptional activation of hox genes during the initiation phase, and the anterior-ward expansion of hox expression domains during the establishment phase. In contrast, in the trunk mesoderm, loss of Cdx4 only delayed the hox initiation phase. Conclusions: These results indicate that Cdx4 differentially regulates the transcription of head, trunk and tail hox genes. In the trunk, Cdx4 conveys spatial positional information to axial tissues primarily by regulating the time of hox gene transcriptional activation during the initiation phase.

AB - Background: Cdx factors expressed in caudal regions of vertebrate embryos regulate hox patterning gene transcription. While loss of Cdx function is known to shift hox spatial expression domains posteriorly, the mechanism underlying the shift is not understood. We addressed this problem by analyzing the spatiotemporal expression profile of all 49 zebrafish hox genes in wild-type and Cdx4-deficient embryos. Results: Loss of Cdx4 had distinct effects on hox spatial expression in a paralogous group-dependent manner: in the head, group 4 expression was expanded posteriorly; in the trunk, group 5-10 expression was shifted posteriorly; and in the tail, group 11-13 genes were expressed in the tail bud but not in more differentiated tissues. In the trunk neural tissue, loss of Cdx4 severely delayed both transcriptional activation of hox genes during the initiation phase, and the anterior-ward expansion of hox expression domains during the establishment phase. In contrast, in the trunk mesoderm, loss of Cdx4 only delayed the hox initiation phase. Conclusions: These results indicate that Cdx4 differentially regulates the transcription of head, trunk and tail hox genes. In the trunk, Cdx4 conveys spatial positional information to axial tissues primarily by regulating the time of hox gene transcriptional activation during the initiation phase.

KW - Axial patterning

KW - Spatial collinearity

KW - Temporal collinearity

UR - http://www.scopus.com/inward/record.url?scp=84983095781&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84983095781&partnerID=8YFLogxK

U2 - 10.1002/dvdy.24343

DO - 10.1002/dvdy.24343

M3 - Article

C2 - 26335559

AN - SCOPUS:84983095781

VL - 244

SP - 1564

EP - 1573

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 12

ER -