Spatial memory in aged rats is related to PKCγ-dependent G-protein coupling of the M1 receptor

M. A. Rossi, D. C. Mash, L. Detoledo-Morrell

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


In the present study, individual differences in spatial memory in aged Fischer 344 (F344) rats were associated with the extent of G-protein coupling of the M1 muscarinic receptor and the dendritic-to-somal ratio of hippocampal PKCγ (d/sPKCγ) immunogenicity. Following testing in the eight-arm radial maze task, 7 young and 13 aged rat brains were sectioned through the dorsal hippocampal formation (HF). G-protein coupling of the M1 receptor was assessed autoradiographically using competition binding studies in the presence and absence of a G-protein uncoupler to determine high (KH) and low (KL) affinity states for agonist in the HF, neocortex, and amygdala. In aged animals, a relationship between choice accuracy in the maze and K H, a measure of M1 receptor-G-protein coupling was seen in the dentate gyrus, CA3, CA1, and neocortex. Furthermore, choice accuracy and d/sPKCγ immunogenicity showed a significant relationship in CA1. Lastly, a correlation was seen in the CA1 of aged animals between KH and d/sPKCγ. These relationships did not hold for the amygdala. Thus, individual differences in a naturally occurring age-dependent disruption of cholinergic-PKCγ signal transduction is associated with spatial memory dysfunction.

Original languageEnglish (US)
Pages (from-to)53-68
Number of pages16
JournalNeurobiology of aging
Issue number1
StatePublished - Jan 2005


  • Aging
  • Amygdala
  • Cholinergic
  • Hippocampus
  • Neocortex
  • Radial arm maze

ASJC Scopus subject areas

  • Clinical Neurology
  • Biological Psychiatry
  • Developmental Neuroscience
  • Neurology
  • Psychology(all)


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