Spatial control of translation repression and polarized growth by conserved NDR kinase Orb6 and RNA-binding protein Sts5

Illyce Nuñez, Marbelys Rodriguez Pino, David J. Wiley, Maitreyi E. Das, Chuan Chen, Tetsuya Goshima, Kazunori Kume, Dai Hirata, Takashi Toda, Fulvia Verde

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

RNA-binding proteins contribute to the formation of ribonucleoprotein (RNP) granules by phase transition, but regulatory mechanisms are not fully understood. Conserved fission yeast NDR (Nuclear Dbf2-Related) kinase Orb6 governs cell morphogenesis in part by spatially controlling Cdc42 GTPase. Here we describe a novel, independent function for Orb6 kinase in negatively regulating the recruitment of RNA-binding protein Sts5 into RNPs to promote polarized cell growth. We find that Orb6 kinase inhibits Sts5 recruitment into granules, its association with processing (P) bodies, and degradation of Sts5-bound mRNAs by promoting Sts5 interaction with 14-3-3 protein Rad24. Many Sts5-bound mRNAs encode essential factors for polarized cell growth, and Orb6 kinase spatially and temporally controls the extent of Sts5 granule formation. Disruption of this control system affects cell morphology and alters the pattern of polarized cell growth, revealing a role for Orb6 kinase in the spatial control of translational repression that enables.

Original languageEnglish (US)
Article numbere14216
JournaleLife
Volume5
Issue numberJULY
DOIs
StatePublished - Jul 30 2016

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RNA-Binding Proteins
Phosphotransferases
Cell growth
Growth
14-3-3 Proteins
Messenger RNA
Ribonucleoproteins
Schizosaccharomyces
GTP Phosphohydrolases
Phase Transition
Morphogenesis
Yeast
Phase transitions
Association reactions
Control systems
Degradation
Processing

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Spatial control of translation repression and polarized growth by conserved NDR kinase Orb6 and RNA-binding protein Sts5. / Nuñez, Illyce; Pino, Marbelys Rodriguez; Wiley, David J.; Das, Maitreyi E.; Chen, Chuan; Goshima, Tetsuya; Kume, Kazunori; Hirata, Dai; Toda, Takashi; Verde, Fulvia.

In: eLife, Vol. 5, No. JULY, e14216, 30.07.2016.

Research output: Contribution to journalArticle

Nuñez, I, Pino, MR, Wiley, DJ, Das, ME, Chen, C, Goshima, T, Kume, K, Hirata, D, Toda, T & Verde, F 2016, 'Spatial control of translation repression and polarized growth by conserved NDR kinase Orb6 and RNA-binding protein Sts5', eLife, vol. 5, no. JULY, e14216. https://doi.org/10.7554/eLife.14216
Nuñez, Illyce ; Pino, Marbelys Rodriguez ; Wiley, David J. ; Das, Maitreyi E. ; Chen, Chuan ; Goshima, Tetsuya ; Kume, Kazunori ; Hirata, Dai ; Toda, Takashi ; Verde, Fulvia. / Spatial control of translation repression and polarized growth by conserved NDR kinase Orb6 and RNA-binding protein Sts5. In: eLife. 2016 ; Vol. 5, No. JULY.
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AU - Chen, Chuan

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