Spatial and temporal gene expression profiling of the contused rat spinal cord

James B. Aimone, J. Leigh Leasure, Victoria M. Perreau, Michaela Thallmair, Aileen J. Anderson, Victor L. Arvanian, Florence M. Bareyre, Mary B Bunge, Carl W. Cotman, Luis Craveiro, Eric D. Crown, V. Reggie Edgerton, Christie Engesser-Cesar, Fred H. Gage, Anthony J. Garcia, Sandra M. Garraway, Philip J. Horner, Susan Howley, Ronaldo M. Ichiyama, Lorne M. MendellLawrence D.F. Moon, Lynne Moore, Luis F. Parada, Damien D Pearse, Jeffrey C. Petruska, Mario I. Romero, Lisa Schnell, Martin E. Schwab, Keith E. Tansey, Sandrine Thuret

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Microarray technology was used to examine gene expression changes following contusive injury of the adult rat spinal cord. To obtain a global understanding of the changes triggered by the injury, differential gene expression was examined spatially, using tissue samples from the epicenter of injury as well as 1 cm rostral and 1 cm caudal to the epicenter, and temporally, at 3 h, 24 h, 7 days, and 35 days post-injury. To filter out gene expression changes that were due to the laminectomy, samples of contused tissue were compared to laminectomy-only controls. We took advantage of four different, complementary methods of data analysis to detect differentially expressed genes. We have identified functional groups of genes that are differentially regulated in our model, including those associated with apoptosis, cell cycle, inflammation, and cholesterol metabolism. Our analysis has led to the identification of novel potential therapeutic targets within each group of genes that is discussed.

Original languageEnglish (US)
Pages (from-to)204-221
Number of pages18
JournalExperimental Neurology
Volume189
Issue number2
DOIs
StatePublished - Oct 1 2004
Externally publishedYes

Fingerprint

Contusions
Gene Expression Profiling
Spinal Cord
Laminectomy
Wounds and Injuries
Gene Expression
Genes
Cell Cycle
Cholesterol
Apoptosis
Inflammation
Technology
Therapeutics

Keywords

  • Affymetrix
  • Apoptosis
  • Cathepsin proteases
  • Cholesterol
  • Contusion
  • Microarray
  • Multi-method analysis

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Aimone, J. B., Leasure, J. L., Perreau, V. M., Thallmair, M., Anderson, A. J., Arvanian, V. L., ... Thuret, S. (2004). Spatial and temporal gene expression profiling of the contused rat spinal cord. Experimental Neurology, 189(2), 204-221. https://doi.org/10.1016/j.expneurol.2004.05.042

Spatial and temporal gene expression profiling of the contused rat spinal cord. / Aimone, James B.; Leasure, J. Leigh; Perreau, Victoria M.; Thallmair, Michaela; Anderson, Aileen J.; Arvanian, Victor L.; Bareyre, Florence M.; Bunge, Mary B; Cotman, Carl W.; Craveiro, Luis; Crown, Eric D.; Edgerton, V. Reggie; Engesser-Cesar, Christie; Gage, Fred H.; Garcia, Anthony J.; Garraway, Sandra M.; Horner, Philip J.; Howley, Susan; Ichiyama, Ronaldo M.; Mendell, Lorne M.; Moon, Lawrence D.F.; Moore, Lynne; Parada, Luis F.; Pearse, Damien D; Petruska, Jeffrey C.; Romero, Mario I.; Schnell, Lisa; Schwab, Martin E.; Tansey, Keith E.; Thuret, Sandrine.

In: Experimental Neurology, Vol. 189, No. 2, 01.10.2004, p. 204-221.

Research output: Contribution to journalArticle

Aimone, JB, Leasure, JL, Perreau, VM, Thallmair, M, Anderson, AJ, Arvanian, VL, Bareyre, FM, Bunge, MB, Cotman, CW, Craveiro, L, Crown, ED, Edgerton, VR, Engesser-Cesar, C, Gage, FH, Garcia, AJ, Garraway, SM, Horner, PJ, Howley, S, Ichiyama, RM, Mendell, LM, Moon, LDF, Moore, L, Parada, LF, Pearse, DD, Petruska, JC, Romero, MI, Schnell, L, Schwab, ME, Tansey, KE & Thuret, S 2004, 'Spatial and temporal gene expression profiling of the contused rat spinal cord', Experimental Neurology, vol. 189, no. 2, pp. 204-221. https://doi.org/10.1016/j.expneurol.2004.05.042
Aimone JB, Leasure JL, Perreau VM, Thallmair M, Anderson AJ, Arvanian VL et al. Spatial and temporal gene expression profiling of the contused rat spinal cord. Experimental Neurology. 2004 Oct 1;189(2):204-221. https://doi.org/10.1016/j.expneurol.2004.05.042
Aimone, James B. ; Leasure, J. Leigh ; Perreau, Victoria M. ; Thallmair, Michaela ; Anderson, Aileen J. ; Arvanian, Victor L. ; Bareyre, Florence M. ; Bunge, Mary B ; Cotman, Carl W. ; Craveiro, Luis ; Crown, Eric D. ; Edgerton, V. Reggie ; Engesser-Cesar, Christie ; Gage, Fred H. ; Garcia, Anthony J. ; Garraway, Sandra M. ; Horner, Philip J. ; Howley, Susan ; Ichiyama, Ronaldo M. ; Mendell, Lorne M. ; Moon, Lawrence D.F. ; Moore, Lynne ; Parada, Luis F. ; Pearse, Damien D ; Petruska, Jeffrey C. ; Romero, Mario I. ; Schnell, Lisa ; Schwab, Martin E. ; Tansey, Keith E. ; Thuret, Sandrine. / Spatial and temporal gene expression profiling of the contused rat spinal cord. In: Experimental Neurology. 2004 ; Vol. 189, No. 2. pp. 204-221.
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