Spatial and temporal gene expression profiling of the contused rat spinal cord

James B. Aimone, J. Leigh Leasure, Victoria M. Perreau, Michaela Thallmair, Aileen J. Anderson, Victor L. Arvanian, Florence M. Bareyre, Mary Bartlett Bunge, Carl W. Cotman, Luis Craveiro, Eric D. Crown, V. Reggie Edgerton, Christie Engesser-Cesar, Fred H. Gage, Anthony J. Garcia, Sandra M. Garraway, Philip J. Horner, Susan Howley, Ronaldo M. Ichiyama, Lorne M. MendellLawrence D.F. Moon, Lynne Moore, Luis F. Parada, Damien D. Pearse, Jeffrey C. Petruska, Mario I. Romero, Lisa Schnell, Martin E. Schwab, Keith E. Tansey, Sandrine Thuret

Research output: Contribution to journalArticlepeer-review

83 Scopus citations

Abstract

Microarray technology was used to examine gene expression changes following contusive injury of the adult rat spinal cord. To obtain a global understanding of the changes triggered by the injury, differential gene expression was examined spatially, using tissue samples from the epicenter of injury as well as 1 cm rostral and 1 cm caudal to the epicenter, and temporally, at 3 h, 24 h, 7 days, and 35 days post-injury. To filter out gene expression changes that were due to the laminectomy, samples of contused tissue were compared to laminectomy-only controls. We took advantage of four different, complementary methods of data analysis to detect differentially expressed genes. We have identified functional groups of genes that are differentially regulated in our model, including those associated with apoptosis, cell cycle, inflammation, and cholesterol metabolism. Our analysis has led to the identification of novel potential therapeutic targets within each group of genes that is discussed.

Original languageEnglish (US)
Pages (from-to)204-221
Number of pages18
JournalExperimental Neurology
Volume189
Issue number2
DOIs
StatePublished - Oct 1 2004

Keywords

  • Affymetrix
  • Apoptosis
  • Cathepsin proteases
  • Cholesterol
  • Contusion
  • Microarray
  • Multi-method analysis

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

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