Sonication-based basic protocol for liposome synthesis

Roberto Mendez, Santanu Banerjee

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Liposomes are spherical vesicles with a wide range of sizes from nano- to micrometer scale. For the past 7–8 decades, these vesicles have occupied the interest of a variety of scientists due to its physical, chemical, and mathematical properties and, to say the least, for its immense utility and potential as delivery vehicles for toxic and nontoxic excipients into biological tissues. Methods related to selection of reagents for creation of specific liposomes of certain properties are beyond the scope of this chapter, but here, we would outline a simplistic protocol to prepare and qualify an uniform batch of simple liposome with basic cargo. This chapter will attempt to provide the reader with a starting point for this immensely potent tool to build upon the right kind of liposome, appropriate for their studies.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages255-260
Number of pages6
DOIs
StatePublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1609
ISSN (Print)1064-3745

Fingerprint

Sonication
Liposomes
Excipients
Poisons

Keywords

  • Drug delivery
  • Lipid
  • Lipid bilayer
  • Liposome
  • LUV
  • Phospholipids
  • Sonication
  • SUV
  • Vesicle

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Mendez, R., & Banerjee, S. (2017). Sonication-based basic protocol for liposome synthesis. In Methods in Molecular Biology (pp. 255-260). (Methods in Molecular Biology; Vol. 1609). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6996-8_21

Sonication-based basic protocol for liposome synthesis. / Mendez, Roberto; Banerjee, Santanu.

Methods in Molecular Biology. Humana Press Inc., 2017. p. 255-260 (Methods in Molecular Biology; Vol. 1609).

Research output: Chapter in Book/Report/Conference proceedingChapter

Mendez, R & Banerjee, S 2017, Sonication-based basic protocol for liposome synthesis. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1609, Humana Press Inc., pp. 255-260. https://doi.org/10.1007/978-1-4939-6996-8_21
Mendez R, Banerjee S. Sonication-based basic protocol for liposome synthesis. In Methods in Molecular Biology. Humana Press Inc. 2017. p. 255-260. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6996-8_21
Mendez, Roberto ; Banerjee, Santanu. / Sonication-based basic protocol for liposome synthesis. Methods in Molecular Biology. Humana Press Inc., 2017. pp. 255-260 (Methods in Molecular Biology).
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