Somatostatin receptors as molecular targets in human uveal melanoma

Kristof Harda, Zsuzsanna Szabo, Erzsebet Szabo, Gabor Olah, Klara Fodor, Csaba Szasz, Gabor Mehes, Andrew V. Schally, Gabor Halmos

Research output: Contribution to journalArticle

Abstract

Uveal melanoma (UM) is the most common primary intraocular malignancy in adults, with an incidence of 4–5 cases per million. The prognosis of UM is very poor. In the present study, our aim was to investigate the expression of mRNA and protein for somatostatin receptor types-1, -2, -3, -4, -5 (SSTR-1–5) in human UM tissue samples and in OCM-1 and OCM-3 human UM cell lines by qRT-PCR, western blot and ligand competition assay. The mRNA for SSTR-2 showed markedly higher expression in UM tissues than SSTR-5. The presence of SSTRs was demonstrated in 70% of UM specimens using ligand competition assay and both human UM models displayed specific high affinity SSTRs. Among the five SSTRs, the mRNA investigated for SSTR-2 and SSTR-5 receptors was strongly expressed in both human UM cell lines, SSTR-5 showing the highest expression. The presence of the SSTR-2 and SSTR-5 receptor proteins was confirmed in both cell lines by western blot. In summary, the expression of somatostatin receptors in human UM specimens and in OCM-1 and OCM-3 human UM cell lines suggests that they could serve as a potential molecular target for therapy of UM using modern powerful cytotoxic SST analogs targeting SSTR-2 and SSTR-5 receptors.

Original languageEnglish (US)
Article number1535;
JournalMolecules
Volume23
Issue number7
DOIs
StatePublished - 2018

Keywords

  • Cancer therapy
  • Human uveal melanoma
  • Molecular targets SSTR-1-5
  • Somatostatin receptors

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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  • Cite this

    Harda, K., Szabo, Z., Szabo, E., Olah, G., Fodor, K., Szasz, C., Mehes, G., Schally, A. V., & Halmos, G. (2018). Somatostatin receptors as molecular targets in human uveal melanoma. Molecules, 23(7), [1535;]. https://doi.org/10.3390/molecules23071535