Somatostatin receptors as molecular targets in human uveal melanoma

Kristof Harda, Zsuzsanna Szabo, Erzsebet Szabo, Gabor Olah, Klara Fodor, Csaba Szasz, Gabor Mehes, Andrew V Schally, Gabor Halmos

Research output: Contribution to journalArticle

Abstract

Uveal melanoma (UM) is the most common primary intraocular malignancy in adults, with an incidence of 4–5 cases per million. The prognosis of UM is very poor. In the present study, our aim was to investigate the expression of mRNA and protein for somatostatin receptor types-1, -2, -3, -4, -5 (SSTR-1–5) in human UM tissue samples and in OCM-1 and OCM-3 human UM cell lines by qRT-PCR, western blot and ligand competition assay. The mRNA for SSTR-2 showed markedly higher expression in UM tissues than SSTR-5. The presence of SSTRs was demonstrated in 70% of UM specimens using ligand competition assay and both human UM models displayed specific high affinity SSTRs. Among the five SSTRs, the mRNA investigated for SSTR-2 and SSTR-5 receptors was strongly expressed in both human UM cell lines, SSTR-5 showing the highest expression. The presence of the SSTR-2 and SSTR-5 receptor proteins was confirmed in both cell lines by western blot. In summary, the expression of somatostatin receptors in human UM specimens and in OCM-1 and OCM-3 human UM cell lines suggests that they could serve as a potential molecular target for therapy of UM using modern powerful cytotoxic SST analogs targeting SSTR-2 and SSTR-5 receptors.

Original languageEnglish (US)
Article number1535;
JournalMolecules
Volume23
Issue number7
DOIs
StatePublished - Jan 1 2018

Fingerprint

Somatostatin Receptors
cultured cells
Cells
Messenger RNA
Assays
Tissue
Ligands
proteins
ligands
Cell Line
prognosis
Proteins
affinity
therapy
incidence
somatostatin receptor 2
Uveal melanoma
analogs
Western Blotting

Keywords

  • Cancer therapy
  • Human uveal melanoma
  • Molecular targets SSTR-1-5
  • Somatostatin receptors

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Harda, K., Szabo, Z., Szabo, E., Olah, G., Fodor, K., Szasz, C., ... Halmos, G. (2018). Somatostatin receptors as molecular targets in human uveal melanoma. Molecules, 23(7), [1535;]. https://doi.org/10.3390/molecules23071535

Somatostatin receptors as molecular targets in human uveal melanoma. / Harda, Kristof; Szabo, Zsuzsanna; Szabo, Erzsebet; Olah, Gabor; Fodor, Klara; Szasz, Csaba; Mehes, Gabor; Schally, Andrew V; Halmos, Gabor.

In: Molecules, Vol. 23, No. 7, 1535;, 01.01.2018.

Research output: Contribution to journalArticle

Harda, K, Szabo, Z, Szabo, E, Olah, G, Fodor, K, Szasz, C, Mehes, G, Schally, AV & Halmos, G 2018, 'Somatostatin receptors as molecular targets in human uveal melanoma', Molecules, vol. 23, no. 7, 1535;. https://doi.org/10.3390/molecules23071535
Harda K, Szabo Z, Szabo E, Olah G, Fodor K, Szasz C et al. Somatostatin receptors as molecular targets in human uveal melanoma. Molecules. 2018 Jan 1;23(7). 1535;. https://doi.org/10.3390/molecules23071535
Harda, Kristof ; Szabo, Zsuzsanna ; Szabo, Erzsebet ; Olah, Gabor ; Fodor, Klara ; Szasz, Csaba ; Mehes, Gabor ; Schally, Andrew V ; Halmos, Gabor. / Somatostatin receptors as molecular targets in human uveal melanoma. In: Molecules. 2018 ; Vol. 23, No. 7.
@article{c3cee190ee0c4761b76b38c73a1ac93a,
title = "Somatostatin receptors as molecular targets in human uveal melanoma",
abstract = "Uveal melanoma (UM) is the most common primary intraocular malignancy in adults, with an incidence of 4–5 cases per million. The prognosis of UM is very poor. In the present study, our aim was to investigate the expression of mRNA and protein for somatostatin receptor types-1, -2, -3, -4, -5 (SSTR-1–5) in human UM tissue samples and in OCM-1 and OCM-3 human UM cell lines by qRT-PCR, western blot and ligand competition assay. The mRNA for SSTR-2 showed markedly higher expression in UM tissues than SSTR-5. The presence of SSTRs was demonstrated in 70{\%} of UM specimens using ligand competition assay and both human UM models displayed specific high affinity SSTRs. Among the five SSTRs, the mRNA investigated for SSTR-2 and SSTR-5 receptors was strongly expressed in both human UM cell lines, SSTR-5 showing the highest expression. The presence of the SSTR-2 and SSTR-5 receptor proteins was confirmed in both cell lines by western blot. In summary, the expression of somatostatin receptors in human UM specimens and in OCM-1 and OCM-3 human UM cell lines suggests that they could serve as a potential molecular target for therapy of UM using modern powerful cytotoxic SST analogs targeting SSTR-2 and SSTR-5 receptors.",
keywords = "Cancer therapy, Human uveal melanoma, Molecular targets SSTR-1-5, Somatostatin receptors",
author = "Kristof Harda and Zsuzsanna Szabo and Erzsebet Szabo and Gabor Olah and Klara Fodor and Csaba Szasz and Gabor Mehes and Schally, {Andrew V} and Gabor Halmos",
year = "2018",
month = "1",
day = "1",
doi = "10.3390/molecules23071535",
language = "English (US)",
volume = "23",
journal = "Molecules",
issn = "1420-3049",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "7",

}

TY - JOUR

T1 - Somatostatin receptors as molecular targets in human uveal melanoma

AU - Harda, Kristof

AU - Szabo, Zsuzsanna

AU - Szabo, Erzsebet

AU - Olah, Gabor

AU - Fodor, Klara

AU - Szasz, Csaba

AU - Mehes, Gabor

AU - Schally, Andrew V

AU - Halmos, Gabor

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Uveal melanoma (UM) is the most common primary intraocular malignancy in adults, with an incidence of 4–5 cases per million. The prognosis of UM is very poor. In the present study, our aim was to investigate the expression of mRNA and protein for somatostatin receptor types-1, -2, -3, -4, -5 (SSTR-1–5) in human UM tissue samples and in OCM-1 and OCM-3 human UM cell lines by qRT-PCR, western blot and ligand competition assay. The mRNA for SSTR-2 showed markedly higher expression in UM tissues than SSTR-5. The presence of SSTRs was demonstrated in 70% of UM specimens using ligand competition assay and both human UM models displayed specific high affinity SSTRs. Among the five SSTRs, the mRNA investigated for SSTR-2 and SSTR-5 receptors was strongly expressed in both human UM cell lines, SSTR-5 showing the highest expression. The presence of the SSTR-2 and SSTR-5 receptor proteins was confirmed in both cell lines by western blot. In summary, the expression of somatostatin receptors in human UM specimens and in OCM-1 and OCM-3 human UM cell lines suggests that they could serve as a potential molecular target for therapy of UM using modern powerful cytotoxic SST analogs targeting SSTR-2 and SSTR-5 receptors.

AB - Uveal melanoma (UM) is the most common primary intraocular malignancy in adults, with an incidence of 4–5 cases per million. The prognosis of UM is very poor. In the present study, our aim was to investigate the expression of mRNA and protein for somatostatin receptor types-1, -2, -3, -4, -5 (SSTR-1–5) in human UM tissue samples and in OCM-1 and OCM-3 human UM cell lines by qRT-PCR, western blot and ligand competition assay. The mRNA for SSTR-2 showed markedly higher expression in UM tissues than SSTR-5. The presence of SSTRs was demonstrated in 70% of UM specimens using ligand competition assay and both human UM models displayed specific high affinity SSTRs. Among the five SSTRs, the mRNA investigated for SSTR-2 and SSTR-5 receptors was strongly expressed in both human UM cell lines, SSTR-5 showing the highest expression. The presence of the SSTR-2 and SSTR-5 receptor proteins was confirmed in both cell lines by western blot. In summary, the expression of somatostatin receptors in human UM specimens and in OCM-1 and OCM-3 human UM cell lines suggests that they could serve as a potential molecular target for therapy of UM using modern powerful cytotoxic SST analogs targeting SSTR-2 and SSTR-5 receptors.

KW - Cancer therapy

KW - Human uveal melanoma

KW - Molecular targets SSTR-1-5

KW - Somatostatin receptors

UR - http://www.scopus.com/inward/record.url?scp=85049408866&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85049408866&partnerID=8YFLogxK

U2 - 10.3390/molecules23071535

DO - 10.3390/molecules23071535

M3 - Article

C2 - 29949880

AN - SCOPUS:85049408866

VL - 23

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 7

M1 - 1535;

ER -