Somatostatin 28(1-12) in a somatostatin-secreting human medullary thyroid carcinoma cell line

David C. Aron, Louis P. Hosta, Elaine B. Vargo, P. C. Andrews, Bernard A. Roos

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

We have identified a system, the TT human medullary thyroid carcinoma cell line, which we found to contain 31.3±27.7 ng of somatostatin 28(1-12) immunoreactivity/mg protein. Radioimmunoassay of gel filtration fractions showed that the major form of immunoreactive somatostatin 28(1-12) had a molecular weight of 1,500 daltons. During reversed-phase high pressure liquid chromatography, this 1,500-dalton species coeluted with synthetic somatostatin 28(1-12). Somatostatin 28(1-12)-containing forms larger than 7,000 daltons were also observed. Further studies will be required to elucidate the route of processing of prosomatostatin. The fact that the products of prosomatostatin processing in these cells are similar to those in normal tissues indicates that the TT medullary thyroid carcinoma cell line constitutes a useful model for human somatostatin gene expression.

Original languageEnglish (US)
Pages (from-to)207-211
Number of pages5
JournalPeptides
Volume8
Issue number2
DOIs
StatePublished - Jan 1 1987

Keywords

  • Medullary thyroid carcinoma
  • Somatostatin
  • Somatostatin 28(1-12)

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

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