Somatostatin 28(1-12) in a somatostatin-secreting human medullary thyroid carcinoma cell line

David C. Aron, Louis P. Hosta, Elaine B. Vargo, P. C. Andrews, Bernard A. Roos

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have identified a system, the TT human medullary thyroid carcinoma cell line, which we found to contain 31.3±27.7 ng of somatostatin 28(1-12) immunoreactivity/mg protein. Radioimmunoassay of gel filtration fractions showed that the major form of immunoreactive somatostatin 28(1-12) had a molecular weight of 1,500 daltons. During reversed-phase high pressure liquid chromatography, this 1,500-dalton species coeluted with synthetic somatostatin 28(1-12). Somatostatin 28(1-12)-containing forms larger than 7,000 daltons were also observed. Further studies will be required to elucidate the route of processing of prosomatostatin. The fact that the products of prosomatostatin processing in these cells are similar to those in normal tissues indicates that the TT medullary thyroid carcinoma cell line constitutes a useful model for human somatostatin gene expression.

Original languageEnglish
Pages (from-to)207-211
Number of pages5
JournalPeptides
Volume8
Issue number2
DOIs
StatePublished - Jan 1 1987
Externally publishedYes

Fingerprint

Somatostatin
Cells
Cell Line
High pressure liquid chromatography
Reverse-Phase Chromatography
Processing
Gene expression
Radioimmunoassay
Gel Chromatography
Molecular Weight
Gels
Molecular weight
High Pressure Liquid Chromatography
Tissue
Gene Expression
somatostatin 28-(1-12)
Medullary Thyroid cancer
Proteins
prosomatostatin

Keywords

  • Medullary thyroid carcinoma
  • Somatostatin
  • Somatostatin 28(1-12)

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Aron, D. C., Hosta, L. P., Vargo, E. B., Andrews, P. C., & Roos, B. A. (1987). Somatostatin 28(1-12) in a somatostatin-secreting human medullary thyroid carcinoma cell line. Peptides, 8(2), 207-211. https://doi.org/10.1016/0196-9781(87)90091-X

Somatostatin 28(1-12) in a somatostatin-secreting human medullary thyroid carcinoma cell line. / Aron, David C.; Hosta, Louis P.; Vargo, Elaine B.; Andrews, P. C.; Roos, Bernard A.

In: Peptides, Vol. 8, No. 2, 01.01.1987, p. 207-211.

Research output: Contribution to journalArticle

Aron, David C. ; Hosta, Louis P. ; Vargo, Elaine B. ; Andrews, P. C. ; Roos, Bernard A. / Somatostatin 28(1-12) in a somatostatin-secreting human medullary thyroid carcinoma cell line. In: Peptides. 1987 ; Vol. 8, No. 2. pp. 207-211.
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