Soluble laminin polymers enhance axon growth of primary neurons in vitro

Agnes E. Haggerty, Hassan Al-Ali, Martin Oudega

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A substrate of laminin polymers formed at pH 4 (acidic pH-induced laminin; aLam) promotes neurite growth of embryonic rat cortical neurons better than a substrate of similar but structurally different laminin polymers formed at neutral pH (neutral pH-induced laminin; nLam). We investigated the effects of these laminin polymers, used as soluble supplements, on neurite growth of cultured adult rat primary dorsal root ganglion neurons. When added to the culture medium, aLam was found to promote neurite growth about twofold better than nLam. Immunoblocking experiments revealed that aLam elicited neurite growth to a similar extent through the α1 or α3 integrin subunit, while nLam required the availability of the α1 integrin subunit to elicit neurite growth. With aLam, but not nLam, immunoblocking of the α1 or α3 subunit resulted in an increase in the protein level of the alternative subunit. The presence of a mature focal adhesion complex, which is associated with neurite growth, was elevated in neurons in the presence of aLam relative to nLam or culture medium. Our data indicated that the two types of laminin polymers promote neurite growth of adult rat primary sensory neurons to a different degree, likely through different ligand-receptor interactions. These findings support the potential of soluble laminin polymers as injectable therapeutics for eliciting axon growth after nervous system injury.

Original languageEnglish (US)
JournalJournal of Biomedical Materials Research - Part A
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Laminin
Neurons
Polymers
Rats
Integrins
Culture Media
Axons
Neurology
Substrates
Adhesion
Ligands
Availability
Proteins

Keywords

  • Dorsal root ganglion
  • Focal adhesion complex
  • Immunoblocking
  • Integrin
  • Neurite extension
  • Vinculin

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

@article{5dc8e4cd243d4680b874318cf87d84af,
title = "Soluble laminin polymers enhance axon growth of primary neurons in vitro",
abstract = "A substrate of laminin polymers formed at pH 4 (acidic pH-induced laminin; aLam) promotes neurite growth of embryonic rat cortical neurons better than a substrate of similar but structurally different laminin polymers formed at neutral pH (neutral pH-induced laminin; nLam). We investigated the effects of these laminin polymers, used as soluble supplements, on neurite growth of cultured adult rat primary dorsal root ganglion neurons. When added to the culture medium, aLam was found to promote neurite growth about twofold better than nLam. Immunoblocking experiments revealed that aLam elicited neurite growth to a similar extent through the α1 or α3 integrin subunit, while nLam required the availability of the α1 integrin subunit to elicit neurite growth. With aLam, but not nLam, immunoblocking of the α1 or α3 subunit resulted in an increase in the protein level of the alternative subunit. The presence of a mature focal adhesion complex, which is associated with neurite growth, was elevated in neurons in the presence of aLam relative to nLam or culture medium. Our data indicated that the two types of laminin polymers promote neurite growth of adult rat primary sensory neurons to a different degree, likely through different ligand-receptor interactions. These findings support the potential of soluble laminin polymers as injectable therapeutics for eliciting axon growth after nervous system injury.",
keywords = "Dorsal root ganglion, Focal adhesion complex, Immunoblocking, Integrin, Neurite extension, Vinculin",
author = "Haggerty, {Agnes E.} and Hassan Al-Ali and Martin Oudega",
year = "2018",
month = "1",
day = "1",
doi = "10.1002/jbm.a.36429",
language = "English (US)",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "1549-3296",
publisher = "Heterocorporation",

}

TY - JOUR

T1 - Soluble laminin polymers enhance axon growth of primary neurons in vitro

AU - Haggerty, Agnes E.

AU - Al-Ali, Hassan

AU - Oudega, Martin

PY - 2018/1/1

Y1 - 2018/1/1

N2 - A substrate of laminin polymers formed at pH 4 (acidic pH-induced laminin; aLam) promotes neurite growth of embryonic rat cortical neurons better than a substrate of similar but structurally different laminin polymers formed at neutral pH (neutral pH-induced laminin; nLam). We investigated the effects of these laminin polymers, used as soluble supplements, on neurite growth of cultured adult rat primary dorsal root ganglion neurons. When added to the culture medium, aLam was found to promote neurite growth about twofold better than nLam. Immunoblocking experiments revealed that aLam elicited neurite growth to a similar extent through the α1 or α3 integrin subunit, while nLam required the availability of the α1 integrin subunit to elicit neurite growth. With aLam, but not nLam, immunoblocking of the α1 or α3 subunit resulted in an increase in the protein level of the alternative subunit. The presence of a mature focal adhesion complex, which is associated with neurite growth, was elevated in neurons in the presence of aLam relative to nLam or culture medium. Our data indicated that the two types of laminin polymers promote neurite growth of adult rat primary sensory neurons to a different degree, likely through different ligand-receptor interactions. These findings support the potential of soluble laminin polymers as injectable therapeutics for eliciting axon growth after nervous system injury.

AB - A substrate of laminin polymers formed at pH 4 (acidic pH-induced laminin; aLam) promotes neurite growth of embryonic rat cortical neurons better than a substrate of similar but structurally different laminin polymers formed at neutral pH (neutral pH-induced laminin; nLam). We investigated the effects of these laminin polymers, used as soluble supplements, on neurite growth of cultured adult rat primary dorsal root ganglion neurons. When added to the culture medium, aLam was found to promote neurite growth about twofold better than nLam. Immunoblocking experiments revealed that aLam elicited neurite growth to a similar extent through the α1 or α3 integrin subunit, while nLam required the availability of the α1 integrin subunit to elicit neurite growth. With aLam, but not nLam, immunoblocking of the α1 or α3 subunit resulted in an increase in the protein level of the alternative subunit. The presence of a mature focal adhesion complex, which is associated with neurite growth, was elevated in neurons in the presence of aLam relative to nLam or culture medium. Our data indicated that the two types of laminin polymers promote neurite growth of adult rat primary sensory neurons to a different degree, likely through different ligand-receptor interactions. These findings support the potential of soluble laminin polymers as injectable therapeutics for eliciting axon growth after nervous system injury.

KW - Dorsal root ganglion

KW - Focal adhesion complex

KW - Immunoblocking

KW - Integrin

KW - Neurite extension

KW - Vinculin

UR - http://www.scopus.com/inward/record.url?scp=85046739594&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046739594&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.36429

DO - 10.1002/jbm.a.36429

M3 - Article

AN - SCOPUS:85046739594

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 1549-3296

ER -