Solid phase synthesis of growth hormone-release inhibiting factor

David H. Coy, Esther J. Coy, Akira Arimura, Andrew V. Schally

Research output: Contribution to journalArticle

77 Scopus citations

Abstract

The growth hormone-release inhibiting factor (GH-RIF) tetradecapeptide {A figure is presented}, was prepared by the cyclization of a linear, disulfhydryl peptide intermediate by treatment with potassium ferricyanide. The linear peptide was synthesized by an automated solid-phase technique. The cyclic material inhibited the secretion of radioimmunoassayable growth hormone in vitro at doses as low as 0.1 μg. A high molecular weight compound formed during the cyclization reaction and believed to be predominantly dimer also possessed considerable inhibitory activity.

Original languageEnglish (US)
Pages (from-to)1267-1273
Number of pages7
JournalBiochemical and biophysical research communications
Volume54
Issue number4
DOIs
StatePublished - Oct 15 1973
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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