Solid phase synthesis of growth hormone-release inhibiting factor

David H. Coy, Esther J. Coy, Akira Arimura, Andrew V Schally

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

The growth hormone-release inhibiting factor (GH-RIF) tetradecapeptide {A figure is presented}, was prepared by the cyclization of a linear, disulfhydryl peptide intermediate by treatment with potassium ferricyanide. The linear peptide was synthesized by an automated solid-phase technique. The cyclic material inhibited the secretion of radioimmunoassayable growth hormone in vitro at doses as low as 0.1 μg. A high molecular weight compound formed during the cyclization reaction and believed to be predominantly dimer also possessed considerable inhibitory activity.

Original languageEnglish
Pages (from-to)1267-1273
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume54
Issue number4
DOIs
StatePublished - Oct 15 1973
Externally publishedYes

Fingerprint

Solid-Phase Synthesis Techniques
Cyclization
Growth Hormone
Peptides
Dimers
Molecular Weight
Molecular weight
potassium ferricyanide
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Solid phase synthesis of growth hormone-release inhibiting factor. / Coy, David H.; Coy, Esther J.; Arimura, Akira; Schally, Andrew V.

In: Biochemical and Biophysical Research Communications, Vol. 54, No. 4, 15.10.1973, p. 1267-1273.

Research output: Contribution to journalArticle

Coy, David H. ; Coy, Esther J. ; Arimura, Akira ; Schally, Andrew V. / Solid phase synthesis of growth hormone-release inhibiting factor. In: Biochemical and Biophysical Research Communications. 1973 ; Vol. 54, No. 4. pp. 1267-1273.
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