Sodium nitroprusside and guanosine 3′,5′-monophosphate (cyclic GMP) inhibit stimulated phosphoinositide hydrolysis in rat cerebral cortical slices

George C. Ormandy, Richard S Jope

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The combination of carbachol and 20 mM K+ greatly stimulates phosphoinositide hydrolysis in rat cerebral cortical slices. This response was inhibited by sodium nitroprusside, an activator of guanylate cyclase which elevated guanosine 3′,5′-monophosphate (cyclic GMP) levels. 8-Bromocyclic GMP and the phorbol ester phorbol myristate acetate (PMA) also inhibited phosphoinositide hydrolysis, and the inhibitory effect of PMA was additive with that of sodium nitroprusside. As guanylate cyclase is Ca2+-dependent this inhibition of phosphoinositide hydrolysis by cyclic GMP may serve as a feedback modulator of the production of inositol phosphates and the ensuing rise of intracellular calcium.

Original languageEnglish
Pages (from-to)287-291
Number of pages5
JournalNeuroscience Letters
Volume100
Issue number1-3
DOIs
StatePublished - May 22 1989
Externally publishedYes

Fingerprint

Guanosine Monophosphate
Nitroprusside
Phosphatidylinositols
Hydrolysis
Guanylate Cyclase
Tetradecanoylphorbol Acetate
Inositol Phosphates
Carbachol
Phorbol Esters
Calcium

Keywords

  • Cyclic GMP
  • Phorbol ester
  • Phosphoinositide hydrolysis
  • Sodium nitroprusside

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

@article{98ce70af0c2a4e9bbfc236710804bbe7,
title = "Sodium nitroprusside and guanosine 3′,5′-monophosphate (cyclic GMP) inhibit stimulated phosphoinositide hydrolysis in rat cerebral cortical slices",
abstract = "The combination of carbachol and 20 mM K+ greatly stimulates phosphoinositide hydrolysis in rat cerebral cortical slices. This response was inhibited by sodium nitroprusside, an activator of guanylate cyclase which elevated guanosine 3′,5′-monophosphate (cyclic GMP) levels. 8-Bromocyclic GMP and the phorbol ester phorbol myristate acetate (PMA) also inhibited phosphoinositide hydrolysis, and the inhibitory effect of PMA was additive with that of sodium nitroprusside. As guanylate cyclase is Ca2+-dependent this inhibition of phosphoinositide hydrolysis by cyclic GMP may serve as a feedback modulator of the production of inositol phosphates and the ensuing rise of intracellular calcium.",
keywords = "Cyclic GMP, Phorbol ester, Phosphoinositide hydrolysis, Sodium nitroprusside",
author = "Ormandy, {George C.} and Jope, {Richard S}",
year = "1989",
month = "5",
day = "22",
doi = "10.1016/0304-3940(89)90700-3",
language = "English",
volume = "100",
pages = "287--291",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1-3",

}

TY - JOUR

T1 - Sodium nitroprusside and guanosine 3′,5′-monophosphate (cyclic GMP) inhibit stimulated phosphoinositide hydrolysis in rat cerebral cortical slices

AU - Ormandy, George C.

AU - Jope, Richard S

PY - 1989/5/22

Y1 - 1989/5/22

N2 - The combination of carbachol and 20 mM K+ greatly stimulates phosphoinositide hydrolysis in rat cerebral cortical slices. This response was inhibited by sodium nitroprusside, an activator of guanylate cyclase which elevated guanosine 3′,5′-monophosphate (cyclic GMP) levels. 8-Bromocyclic GMP and the phorbol ester phorbol myristate acetate (PMA) also inhibited phosphoinositide hydrolysis, and the inhibitory effect of PMA was additive with that of sodium nitroprusside. As guanylate cyclase is Ca2+-dependent this inhibition of phosphoinositide hydrolysis by cyclic GMP may serve as a feedback modulator of the production of inositol phosphates and the ensuing rise of intracellular calcium.

AB - The combination of carbachol and 20 mM K+ greatly stimulates phosphoinositide hydrolysis in rat cerebral cortical slices. This response was inhibited by sodium nitroprusside, an activator of guanylate cyclase which elevated guanosine 3′,5′-monophosphate (cyclic GMP) levels. 8-Bromocyclic GMP and the phorbol ester phorbol myristate acetate (PMA) also inhibited phosphoinositide hydrolysis, and the inhibitory effect of PMA was additive with that of sodium nitroprusside. As guanylate cyclase is Ca2+-dependent this inhibition of phosphoinositide hydrolysis by cyclic GMP may serve as a feedback modulator of the production of inositol phosphates and the ensuing rise of intracellular calcium.

KW - Cyclic GMP

KW - Phorbol ester

KW - Phosphoinositide hydrolysis

KW - Sodium nitroprusside

UR - http://www.scopus.com/inward/record.url?scp=0024519180&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024519180&partnerID=8YFLogxK

U2 - 10.1016/0304-3940(89)90700-3

DO - 10.1016/0304-3940(89)90700-3

M3 - Article

C2 - 2548129

AN - SCOPUS:0024519180

VL - 100

SP - 287

EP - 291

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 1-3

ER -