Sodium nitroprusside and guanosine 3′,5′-monophosphate (cyclic GMP) inhibit stimulated phosphoinositide hydrolysis in rat cerebral cortical slices

George C. Ormandy; Richard S. Jope

doi:10.1016/0304-3940(89)90700-3

Sodium nitroprusside and guanosine 3′,5′-monophosphate (cyclic GMP) inhibit stimulated phosphoinositide hydrolysis in rat cerebral cortical slices

George C. Ormandy, Richard S. Jope

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The combination of carbachol and 20 mM K⁺ greatly stimulates phosphoinositide hydrolysis in rat cerebral cortical slices. This response was inhibited by sodium nitroprusside, an activator of guanylate cyclase which elevated guanosine 3′,5′-monophosphate (cyclic GMP) levels. 8-Bromocyclic GMP and the phorbol ester phorbol myristate acetate (PMA) also inhibited phosphoinositide hydrolysis, and the inhibitory effect of PMA was additive with that of sodium nitroprusside. As guanylate cyclase is Ca²⁺-dependent this inhibition of phosphoinositide hydrolysis by cyclic GMP may serve as a feedback modulator of the production of inositol phosphates and the ensuing rise of intracellular calcium.

Original language	English (US)
Pages (from-to)	287-291
Number of pages	5
Journal	Neuroscience Letters
Volume	100
Issue number	1-3
DOIs	https://doi.org/10.1016/0304-3940(89)90700-3
State	Published - May 22 1989
Externally published	Yes

Keywords

Cyclic GMP
Phorbol ester
Phosphoinositide hydrolysis
Sodium nitroprusside

ASJC Scopus subject areas

Neuroscience(all)

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title = "Sodium nitroprusside and guanosine 3′,5′-monophosphate (cyclic GMP) inhibit stimulated phosphoinositide hydrolysis in rat cerebral cortical slices",

abstract = "The combination of carbachol and 20 mM K+ greatly stimulates phosphoinositide hydrolysis in rat cerebral cortical slices. This response was inhibited by sodium nitroprusside, an activator of guanylate cyclase which elevated guanosine 3′,5′-monophosphate (cyclic GMP) levels. 8-Bromocyclic GMP and the phorbol ester phorbol myristate acetate (PMA) also inhibited phosphoinositide hydrolysis, and the inhibitory effect of PMA was additive with that of sodium nitroprusside. As guanylate cyclase is Ca2+-dependent this inhibition of phosphoinositide hydrolysis by cyclic GMP may serve as a feedback modulator of the production of inositol phosphates and the ensuing rise of intracellular calcium.",

keywords = "Cyclic GMP, Phorbol ester, Phosphoinositide hydrolysis, Sodium nitroprusside",

author = "Ormandy, {George C.} and Jope, {Richard S.}",

note = "Funding Information: The authors thank Diane Lee for manuscript preparation. This work was supported by grants from NIMH (MH 38752) and NIH (NS 26165).",

year = "1989",

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doi = "10.1016/0304-3940(89)90700-3",

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T1 - Sodium nitroprusside and guanosine 3′,5′-monophosphate (cyclic GMP) inhibit stimulated phosphoinositide hydrolysis in rat cerebral cortical slices

AU - Ormandy, George C.

AU - Jope, Richard S.

N1 - Funding Information: The authors thank Diane Lee for manuscript preparation. This work was supported by grants from NIMH (MH 38752) and NIH (NS 26165).

PY - 1989/5/22

Y1 - 1989/5/22

N2 - The combination of carbachol and 20 mM K+ greatly stimulates phosphoinositide hydrolysis in rat cerebral cortical slices. This response was inhibited by sodium nitroprusside, an activator of guanylate cyclase which elevated guanosine 3′,5′-monophosphate (cyclic GMP) levels. 8-Bromocyclic GMP and the phorbol ester phorbol myristate acetate (PMA) also inhibited phosphoinositide hydrolysis, and the inhibitory effect of PMA was additive with that of sodium nitroprusside. As guanylate cyclase is Ca2+-dependent this inhibition of phosphoinositide hydrolysis by cyclic GMP may serve as a feedback modulator of the production of inositol phosphates and the ensuing rise of intracellular calcium.

AB - The combination of carbachol and 20 mM K+ greatly stimulates phosphoinositide hydrolysis in rat cerebral cortical slices. This response was inhibited by sodium nitroprusside, an activator of guanylate cyclase which elevated guanosine 3′,5′-monophosphate (cyclic GMP) levels. 8-Bromocyclic GMP and the phorbol ester phorbol myristate acetate (PMA) also inhibited phosphoinositide hydrolysis, and the inhibitory effect of PMA was additive with that of sodium nitroprusside. As guanylate cyclase is Ca2+-dependent this inhibition of phosphoinositide hydrolysis by cyclic GMP may serve as a feedback modulator of the production of inositol phosphates and the ensuing rise of intracellular calcium.

KW - Cyclic GMP

KW - Phorbol ester

KW - Phosphoinositide hydrolysis

KW - Sodium nitroprusside

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Sodium nitroprusside and guanosine 3′,5′-monophosphate (cyclic GMP) inhibit stimulated phosphoinositide hydrolysis in rat cerebral cortical slices

Abstract

Keywords

ASJC Scopus subject areas

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