Small nuclear RNAs encoded by Herpesvirus saimiri upregulate the expression of genes linked to T cell activation in virally transformed T cells

Heidi L. Cook, J. Robin Lytle, Hannah E. Mischo, Ming Jie Li, John J. Rossi, Daniel P. Silva, Ronald Charles Desrosiers, Joan A. Steitz

Research output: Contribution to journalArticle

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Abstract

Seven small nuclear RNAs of the Sm class are encoded by Herpesvirus saimiri (HVS), a γ Herpesvirus that causes aggressive T cell leukemias and lymphomas in New World primates and efficiently transforms T cells in vitro [1-4]. The Herpesvirus saimiri U RNAs (HSURs) are the most abundant viral transcripts in HVS-transformed, latently infected T cells but are not required for viral replication or transformation in vitro [5]. We have compared marmoset T cells transformed with wild-type or a mutant HVS lacking the most highly conserved HSURs, HSURs 1 and 2. Microarray and Northern analyses reveal that HSUR 1 and 2 expression correlates with significant increases in a small number of host mRNAs, including the T cell-receptor β and γ chains, the T cell and natural killer (NK) cell-surface receptors CD52 and DAP10, and intracellular proteins - SKAP55, granulysin, and NKG7 - linked to T cell and NK cell activation. Upregulation of three of these transcripts was rescued after transduction of deletion-mutant-HVS-transformed cells with a lentiviral vector carrying HSURs 1 and 2. These changes indicate an unexpected role for the HSURs in regulating a remarkably defined and physiologically relevant set of host targets involved in the activation of virally transformed T cells during latency.

Original languageEnglish (US)
Pages (from-to)974-979
Number of pages6
JournalCurrent Biology
Volume15
Issue number10
DOIs
StatePublished - May 24 2005
Externally publishedYes

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Saimiriine herpesvirus 2
Saimiriine Herpesvirus 2
small nuclear RNA
Small Nuclear RNA
T-cells
Up-Regulation
T-lymphocytes
Genes
Chemical activation
T-Lymphocytes
Gene Expression
gene expression
RNA
natural killer cells
Cell Surface Receptors
Microarrays
T-Cell Antigen Receptor
Natural Killer Cell Receptors
T-Cell Leukemia
Callithrix

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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Small nuclear RNAs encoded by Herpesvirus saimiri upregulate the expression of genes linked to T cell activation in virally transformed T cells. / Cook, Heidi L.; Lytle, J. Robin; Mischo, Hannah E.; Li, Ming Jie; Rossi, John J.; Silva, Daniel P.; Desrosiers, Ronald Charles; Steitz, Joan A.

In: Current Biology, Vol. 15, No. 10, 24.05.2005, p. 974-979.

Research output: Contribution to journalArticle

Cook, Heidi L. ; Lytle, J. Robin ; Mischo, Hannah E. ; Li, Ming Jie ; Rossi, John J. ; Silva, Daniel P. ; Desrosiers, Ronald Charles ; Steitz, Joan A. / Small nuclear RNAs encoded by Herpesvirus saimiri upregulate the expression of genes linked to T cell activation in virally transformed T cells. In: Current Biology. 2005 ; Vol. 15, No. 10. pp. 974-979.
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