Small animal ocular biometry using optical coherence tomography

Marco Ruggeri, Omer Kocaoglu, Stephen Uhlhorn, David Borja, Raksha Urs, Tsung Han Chou, Vittorio Porciatti, Jean Marie Parel, Fabrice Manns

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

A custom-built OCT system was used to obtain images of the whole mouse eye. We developed a semi-automated segmentation method to detect the boundaries of the anterior and posterior corneal, lens and retinal surfaces as well as the anterior surface of the iris. The radii of curvature of the surfaces were calculated using a conic section fit of each boundary. Image distortions due to refraction of the OCT beam at the successive boundaries were corrected using a ray-tracing algorithm. Corrected ocular distances, radii of curvature of the cornea and lens surfaces, and anterior chamber angle were obtained on 3 C57BL/6J mice. In vivo imaging of the whole eye, segmentation, conic function fits and correction were successful in all three animals. The posterior lens surface of one mouse could not be fit accurately with a conic section. Biometric parameters of C57BL/6J mice compared well with previous published data obtained from histological sections. The study demonstrates the feasibility of quantitative in vivo biometry of mouse models.

Original languageEnglish (US)
Title of host publicationOphthalmic Technologies XX
DOIs
StatePublished - Dec 1 2010
EventOphthalmic Technologies XX - San Francisco, CA, United States
Duration: Jan 23 2010Jan 25 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7550
ISSN (Print)1605-7422

Other

OtherOphthalmic Technologies XX
CountryUnited States
CitySan Francisco, CA
Period1/23/101/25/10

Keywords

  • Imaging
  • Optical coherence tomography
  • Refraction correction

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Ruggeri, M., Kocaoglu, O., Uhlhorn, S., Borja, D., Urs, R., Chou, T. H., Porciatti, V., Parel, J. M., & Manns, F. (2010). Small animal ocular biometry using optical coherence tomography. In Ophthalmic Technologies XX [755016] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7550). https://doi.org/10.1117/12.846937