Small animal ocular biometry using optical coherence tomography

Marco Ruggeri, Omer Kocaoglu, Stephen Uhlhorn, David Borja, Raksha Urs, Tsung Han Chou, Vittorio Porciatti, Jean-Marie A Parel, Fabrice Manns

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

A custom-built OCT system was used to obtain images of the whole mouse eye. We developed a semi-automated segmentation method to detect the boundaries of the anterior and posterior corneal, lens and retinal surfaces as well as the anterior surface of the iris. The radii of curvature of the surfaces were calculated using a conic section fit of each boundary. Image distortions due to refraction of the OCT beam at the successive boundaries were corrected using a ray-tracing algorithm. Corrected ocular distances, radii of curvature of the cornea and lens surfaces, and anterior chamber angle were obtained on 3 C57BL/6J mice. In vivo imaging of the whole eye, segmentation, conic function fits and correction were successful in all three animals. The posterior lens surface of one mouse could not be fit accurately with a conic section. Biometric parameters of C57BL/6J mice compared well with previous published data obtained from histological sections. The study demonstrates the feasibility of quantitative in vivo biometry of mouse models.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7550
DOIs
StatePublished - Dec 1 2010
EventOphthalmic Technologies XX - San Francisco, CA, United States
Duration: Jan 23 2010Jan 25 2010

Other

OtherOphthalmic Technologies XX
CountryUnited States
CitySan Francisco, CA
Period1/23/101/25/10

Fingerprint

Biometry
Optical tomography
Optical Coherence Tomography
Lenses
mice
animals
Animals
tomography
Inbred C57BL Mouse
lenses
Feasibility Studies
Anterior Chamber
Iris
Cornea
curvature
biometrics
radii
cornea
Ray tracing
Biometrics

Keywords

  • Imaging
  • Optical coherence tomography
  • Refraction correction

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Ruggeri, M., Kocaoglu, O., Uhlhorn, S., Borja, D., Urs, R., Chou, T. H., ... Manns, F. (2010). Small animal ocular biometry using optical coherence tomography. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7550). [755016] https://doi.org/10.1117/12.846937

Small animal ocular biometry using optical coherence tomography. / Ruggeri, Marco; Kocaoglu, Omer; Uhlhorn, Stephen; Borja, David; Urs, Raksha; Chou, Tsung Han; Porciatti, Vittorio; Parel, Jean-Marie A; Manns, Fabrice.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7550 2010. 755016.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ruggeri, M, Kocaoglu, O, Uhlhorn, S, Borja, D, Urs, R, Chou, TH, Porciatti, V, Parel, J-MA & Manns, F 2010, Small animal ocular biometry using optical coherence tomography. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7550, 755016, Ophthalmic Technologies XX, San Francisco, CA, United States, 1/23/10. https://doi.org/10.1117/12.846937
Ruggeri M, Kocaoglu O, Uhlhorn S, Borja D, Urs R, Chou TH et al. Small animal ocular biometry using optical coherence tomography. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7550. 2010. 755016 https://doi.org/10.1117/12.846937
Ruggeri, Marco ; Kocaoglu, Omer ; Uhlhorn, Stephen ; Borja, David ; Urs, Raksha ; Chou, Tsung Han ; Porciatti, Vittorio ; Parel, Jean-Marie A ; Manns, Fabrice. / Small animal ocular biometry using optical coherence tomography. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7550 2010.
@inproceedings{da8957955ef24ea3a60e6ec15267858a,
title = "Small animal ocular biometry using optical coherence tomography",
abstract = "A custom-built OCT system was used to obtain images of the whole mouse eye. We developed a semi-automated segmentation method to detect the boundaries of the anterior and posterior corneal, lens and retinal surfaces as well as the anterior surface of the iris. The radii of curvature of the surfaces were calculated using a conic section fit of each boundary. Image distortions due to refraction of the OCT beam at the successive boundaries were corrected using a ray-tracing algorithm. Corrected ocular distances, radii of curvature of the cornea and lens surfaces, and anterior chamber angle were obtained on 3 C57BL/6J mice. In vivo imaging of the whole eye, segmentation, conic function fits and correction were successful in all three animals. The posterior lens surface of one mouse could not be fit accurately with a conic section. Biometric parameters of C57BL/6J mice compared well with previous published data obtained from histological sections. The study demonstrates the feasibility of quantitative in vivo biometry of mouse models.",
keywords = "Imaging, Optical coherence tomography, Refraction correction",
author = "Marco Ruggeri and Omer Kocaoglu and Stephen Uhlhorn and David Borja and Raksha Urs and Chou, {Tsung Han} and Vittorio Porciatti and Parel, {Jean-Marie A} and Fabrice Manns",
year = "2010",
month = "12",
day = "1",
doi = "10.1117/12.846937",
language = "English",
isbn = "9780819479464",
volume = "7550",
booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

}

TY - GEN

T1 - Small animal ocular biometry using optical coherence tomography

AU - Ruggeri, Marco

AU - Kocaoglu, Omer

AU - Uhlhorn, Stephen

AU - Borja, David

AU - Urs, Raksha

AU - Chou, Tsung Han

AU - Porciatti, Vittorio

AU - Parel, Jean-Marie A

AU - Manns, Fabrice

PY - 2010/12/1

Y1 - 2010/12/1

N2 - A custom-built OCT system was used to obtain images of the whole mouse eye. We developed a semi-automated segmentation method to detect the boundaries of the anterior and posterior corneal, lens and retinal surfaces as well as the anterior surface of the iris. The radii of curvature of the surfaces were calculated using a conic section fit of each boundary. Image distortions due to refraction of the OCT beam at the successive boundaries were corrected using a ray-tracing algorithm. Corrected ocular distances, radii of curvature of the cornea and lens surfaces, and anterior chamber angle were obtained on 3 C57BL/6J mice. In vivo imaging of the whole eye, segmentation, conic function fits and correction were successful in all three animals. The posterior lens surface of one mouse could not be fit accurately with a conic section. Biometric parameters of C57BL/6J mice compared well with previous published data obtained from histological sections. The study demonstrates the feasibility of quantitative in vivo biometry of mouse models.

AB - A custom-built OCT system was used to obtain images of the whole mouse eye. We developed a semi-automated segmentation method to detect the boundaries of the anterior and posterior corneal, lens and retinal surfaces as well as the anterior surface of the iris. The radii of curvature of the surfaces were calculated using a conic section fit of each boundary. Image distortions due to refraction of the OCT beam at the successive boundaries were corrected using a ray-tracing algorithm. Corrected ocular distances, radii of curvature of the cornea and lens surfaces, and anterior chamber angle were obtained on 3 C57BL/6J mice. In vivo imaging of the whole eye, segmentation, conic function fits and correction were successful in all three animals. The posterior lens surface of one mouse could not be fit accurately with a conic section. Biometric parameters of C57BL/6J mice compared well with previous published data obtained from histological sections. The study demonstrates the feasibility of quantitative in vivo biometry of mouse models.

KW - Imaging

KW - Optical coherence tomography

KW - Refraction correction

UR - http://www.scopus.com/inward/record.url?scp=79953065068&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79953065068&partnerID=8YFLogxK

U2 - 10.1117/12.846937

DO - 10.1117/12.846937

M3 - Conference contribution

SN - 9780819479464

VL - 7550

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

ER -