Sm21.6 a novel EF-hand family protein member located on the surface of Schistosoma mansoni adult worm that failed to induce protection against challenge infection but reduced liver pathology

Debora O. Lopes, Leonardo F. Paiva, Mauricio A. Martins, Fernanda C. Cardoso, Matheus A. Rajão, Jean M. Pinho, Marcelo V. Caliari, Rodrigo Correa-Oliveira, Samantha M. Mello, Luciana C.C. Leite, Sergio C. Oliveira

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Schistosomiasis continues to be a significant public health problem that affects 200 million people worldwide. This is one of the most important parasitic diseases, and one whose effective control is unlikely in the absence of a vaccine. In this study, we have isolated a cDNA clone encoding the Schistosoma mansoni Sm21.6 protein that has 45% and 44% identity with Sm22.6 and Sj21.7 EF-hand containing antigens, respectively. Confocal microscopy analysis revealed that Sm21.6 is a membrane-associated protein localized on the S. mansoni adult worm. Mouse immunization with rSm21.6 induced a mixed Th1/Th2 cytokine profile and no protection against infection. However, vaccination with rSm21.6 reduced by 28% of liver granuloma numbers, 21% of granuloma area and 34% of fibrosis. Finally, rSm21.6 was recognized by sera from individuals resistant to reinfection compared with patients susceptible to reinfection and this molecule should be further studied as potential biomarker for disease resistance. In conclusion, Sm21.6 is a new tegument protein from S. mansoni that plays an important role in reducing pathology induced by parasite infection.

Original languageEnglish (US)
Pages (from-to)4127-4135
Number of pages9
JournalVaccine
Volume27
Issue number31
DOIs
StatePublished - Jun 24 2009
Externally publishedYes

Keywords

  • Cytokines
  • Recombinant protein
  • Schistosoma mansoni
  • Sm21.6
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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    Lopes, D. O., Paiva, L. F., Martins, M. A., Cardoso, F. C., Rajão, M. A., Pinho, J. M., Caliari, M. V., Correa-Oliveira, R., Mello, S. M., Leite, L. C. C., & Oliveira, S. C. (2009). Sm21.6 a novel EF-hand family protein member located on the surface of Schistosoma mansoni adult worm that failed to induce protection against challenge infection but reduced liver pathology. Vaccine, 27(31), 4127-4135. https://doi.org/10.1016/j.vaccine.2009.04.068