Slow release iodine preparation and wound healing: In vitro effects consistent with lack of in vivo toxicity in human chronic wounds

L. H. Zhou, W. K. Nahm, Evangelos V Badiavas, T. Yufit, V. Falanga

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Background: Antiseptic agents, particularly slow-release preparations, are increasingly being used in the management of chronic wounds. One such agent, cadexomer iodine, carries iodine (0.9% weight/weight) immobilized in beads of dextrin and epichlorhydrin and has been demonstrated to be highly effective in promoting healing of exudative wounds. However, there have been no studies directly assessing the potential lack of toxicity of cadexomer iodine on human cutaneous tissues. Objectives: To determine if, within a certain concentration range, cadexomer iodine is non-toxic to human cells and cutaneous tissue and to assess histologically human chronic exudative wounds that are being treated with cadexomer iodine. Methods: We examined the effects of varying concentrations of cadexomer iodine on the viability of human fibroblasts in culture (by trypan blue exclusion). The morphology, cellular proliferation capacity (measured by [3H]thymidine uptake), ability to produce α1(I) procollagen chain mRNA, and cell outgrowth from neonatal foreskin explants were also evaluated in human fibroblasts after incubation with various concentrations of cadexomer iodine. Moreover, biopsies of chronic exudative wounds concurrently treated with cadexomer iodine were stained with haematoxylin and eosin or a Gram stain and evaluated microscopically. Results: At concentrations of up to 0.45%, cadexomer iodine was found to be non-toxic to fibroblasts in vitro; there were no changes in viability, morphology, cellular proliferation, ability to produce collagen, and cell outgrowth from explants. In vivo, skin biopsies of chronic exudative wounds being treated with cadexomer iodine demonstrated no evidence of cell necrosis, displayed re-epithelialization, and revealed bacteria within the cadexomer beads. Conclusions: These studies demonstrate that cadexomer iodine has definite non-toxic concentration ranges for fibroblasts in vitro, which are consistent with a lack of cellular toxicity in human chronic exudative wounds treated with cadexomer iodine. Cadexomer iodine may also have the additional property of trapping microorganisms.

Original languageEnglish
Pages (from-to)365-374
Number of pages10
JournalBritish Journal of Dermatology
Volume146
Issue number3
DOIs
StatePublished - Jun 18 2002
Externally publishedYes

Fingerprint

Iodine
Wound Healing
Wounds and Injuries
Fibroblasts
Skin
cadexomer iodine
In Vitro Techniques
Epichlorohydrin
Cell Proliferation
Re-Epithelialization
Biopsy
Foreskin
Weights and Measures
Procollagen
Local Anti-Infective Agents
Trypan Blue
Hematoxylin
Eosine Yellowish-(YS)
Thymidine
Necrosis

Keywords

  • Antiseptic
  • Cadexomer iodine
  • Collagen
  • Fibroblast
  • Thymidine uptake
  • Wounds

ASJC Scopus subject areas

  • Dermatology

Cite this

Slow release iodine preparation and wound healing : In vitro effects consistent with lack of in vivo toxicity in human chronic wounds. / Zhou, L. H.; Nahm, W. K.; Badiavas, Evangelos V; Yufit, T.; Falanga, V.

In: British Journal of Dermatology, Vol. 146, No. 3, 18.06.2002, p. 365-374.

Research output: Contribution to journalArticle

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abstract = "Background: Antiseptic agents, particularly slow-release preparations, are increasingly being used in the management of chronic wounds. One such agent, cadexomer iodine, carries iodine (0.9{\%} weight/weight) immobilized in beads of dextrin and epichlorhydrin and has been demonstrated to be highly effective in promoting healing of exudative wounds. However, there have been no studies directly assessing the potential lack of toxicity of cadexomer iodine on human cutaneous tissues. Objectives: To determine if, within a certain concentration range, cadexomer iodine is non-toxic to human cells and cutaneous tissue and to assess histologically human chronic exudative wounds that are being treated with cadexomer iodine. Methods: We examined the effects of varying concentrations of cadexomer iodine on the viability of human fibroblasts in culture (by trypan blue exclusion). The morphology, cellular proliferation capacity (measured by [3H]thymidine uptake), ability to produce α1(I) procollagen chain mRNA, and cell outgrowth from neonatal foreskin explants were also evaluated in human fibroblasts after incubation with various concentrations of cadexomer iodine. Moreover, biopsies of chronic exudative wounds concurrently treated with cadexomer iodine were stained with haematoxylin and eosin or a Gram stain and evaluated microscopically. Results: At concentrations of up to 0.45{\%}, cadexomer iodine was found to be non-toxic to fibroblasts in vitro; there were no changes in viability, morphology, cellular proliferation, ability to produce collagen, and cell outgrowth from explants. In vivo, skin biopsies of chronic exudative wounds being treated with cadexomer iodine demonstrated no evidence of cell necrosis, displayed re-epithelialization, and revealed bacteria within the cadexomer beads. Conclusions: These studies demonstrate that cadexomer iodine has definite non-toxic concentration ranges for fibroblasts in vitro, which are consistent with a lack of cellular toxicity in human chronic exudative wounds treated with cadexomer iodine. Cadexomer iodine may also have the additional property of trapping microorganisms.",
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AU - Yufit, T.

AU - Falanga, V.

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