Slitlamp photography and videography with high magnifications

Jin Yuan, Hong Jiang, Xinjie Mao, Bilian Ke, Wentao Yan, Che Liu, Hector R. Cintrón-Colón, Victor L Perez Quinones, Jianhua Wang

Research output: Contribution to journalArticle

Abstract

Purpose: To demonstrate the use of the slitlamp photography and videography with extremely high magnifications for visualizing structures of the anterior segment of the eye. Methods: A Canon 60D digital camera with Movie Crop Function was adapted into a Nikon FS-2 slitlamp to capture still images and video clips of the structures of the anterior segment of the eye. Images obtained using the slitlamp were tested for spatial resolution. The cornea of human eyes was imaged with the slitlamp, and the structures were compared with the pictures captured using the ultra-high-resolution optical coherence tomography (UHR-OCT). The central thickness of the corneal epithelium and total cornea was obtained using the slitlamp, and the results were compared with the thickness obtained using UHR-OCT. Results: High-quality ocular images and higher spatial resolutions were obtained using the slitlamp with extremely high magnifications and Movie Crop Function, rather than the traditional slitlamp. The structures and characteristics of the cornea, such as the normal epithelium, abnormal epithelium of corneal intraepithelial neoplasia, laser in situ keratomileusis interface, and contact lenses, were clearly visualized using this device. These features were confirmed by comparing the obtained images with those acquired using UHR-OCT. Moreover, the tear film debris on the ocular surface and the corneal nerve in the anterior corneal stroma were also visualized. The thicknesses of the corneal epithelium and total cornea were similar to that measured using UHR-OCT (P

Original languageEnglish (US)
Pages (from-to)391-397
Number of pages7
JournalEye and Contact Lens
Volume41
Issue number6
DOIs
StatePublished - 2015

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Photography
Optical Coherence Tomography
Cornea
Corneal Epithelium
Anterior Eye Segment
Motion Pictures
Corneal Stroma
Laser In Situ Keratomileusis
Contact Lenses
Tears
Surgical Instruments
Epithelium
Equipment and Supplies
Neoplasms

Keywords

  • Corneal epithelial thickness
  • High magnification
  • Slitlamp biomicroscopy
  • Ultra-high optical coherence topography
  • Videography

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Slitlamp photography and videography with high magnifications. / Yuan, Jin; Jiang, Hong; Mao, Xinjie; Ke, Bilian; Yan, Wentao; Liu, Che; Cintrón-Colón, Hector R.; Perez Quinones, Victor L; Wang, Jianhua.

In: Eye and Contact Lens, Vol. 41, No. 6, 2015, p. 391-397.

Research output: Contribution to journalArticle

Yuan, J, Jiang, H, Mao, X, Ke, B, Yan, W, Liu, C, Cintrón-Colón, HR, Perez Quinones, VL & Wang, J 2015, 'Slitlamp photography and videography with high magnifications', Eye and Contact Lens, vol. 41, no. 6, pp. 391-397. https://doi.org/10.1097/ICL.0000000000000148
Yuan, Jin ; Jiang, Hong ; Mao, Xinjie ; Ke, Bilian ; Yan, Wentao ; Liu, Che ; Cintrón-Colón, Hector R. ; Perez Quinones, Victor L ; Wang, Jianhua. / Slitlamp photography and videography with high magnifications. In: Eye and Contact Lens. 2015 ; Vol. 41, No. 6. pp. 391-397.
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