Abstract
Purpose: To demonstrate the use of the slitlamp photography and videography with extremely high magnifications for visualizing structures of the anterior segment of the eye. Methods: A Canon 60D digital camera with Movie Crop Function was adapted into a Nikon FS-2 slitlamp to capture still images and video clips of the structures of the anterior segment of the eye. Images obtained using the slitlamp were tested for spatial resolution. The cornea of human eyes was imaged with the slitlamp, and the structures were compared with the pictures captured using the ultra-high-resolution optical coherence tomography (UHR-OCT). The central thickness of the corneal epithelium and total cornea was obtained using the slitlamp, and the results were compared with the thickness obtained using UHR-OCT. Results: High-quality ocular images and higher spatial resolutions were obtained using the slitlamp with extremely high magnifications and Movie Crop Function, rather than the traditional slitlamp. The structures and characteristics of the cornea, such as the normal epithelium, abnormal epithelium of corneal intraepithelial neoplasia, laser in situ keratomileusis interface, and contact lenses, were clearly visualized using this device. These features were confirmed by comparing the obtained images with those acquired using UHR-OCT. Moreover, the tear film debris on the ocular surface and the corneal nerve in the anterior corneal stroma were also visualized. The thicknesses of the corneal epithelium and total cornea were similar to that measured using UHR-OCT (P
| Original language | English (US) |
|---|---|
| Pages (from-to) | 391-397 |
| Number of pages | 7 |
| Journal | Eye and Contact Lens |
| Volume | 41 |
| Issue number | 6 |
| DOIs | |
| State | Published - 2015 |
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Keywords
- Corneal epithelial thickness
- High magnification
- Slitlamp biomicroscopy
- Ultra-high optical coherence topography
- Videography
ASJC Scopus subject areas
- Ophthalmology
Cite this
Slitlamp photography and videography with high magnifications. / Yuan, Jin; Jiang, Hong; Mao, Xinjie; Ke, Bilian; Yan, Wentao; Liu, Che; Cintrón-Colón, Hector R.; Perez Quinones, Victor L; Wang, Jianhua.
In: Eye and Contact Lens, Vol. 41, No. 6, 2015, p. 391-397.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Slitlamp photography and videography with high magnifications
AU - Yuan, Jin
AU - Jiang, Hong
AU - Mao, Xinjie
AU - Ke, Bilian
AU - Yan, Wentao
AU - Liu, Che
AU - Cintrón-Colón, Hector R.
AU - Perez Quinones, Victor L
AU - Wang, Jianhua
PY - 2015
Y1 - 2015
N2 - Purpose: To demonstrate the use of the slitlamp photography and videography with extremely high magnifications for visualizing structures of the anterior segment of the eye. Methods: A Canon 60D digital camera with Movie Crop Function was adapted into a Nikon FS-2 slitlamp to capture still images and video clips of the structures of the anterior segment of the eye. Images obtained using the slitlamp were tested for spatial resolution. The cornea of human eyes was imaged with the slitlamp, and the structures were compared with the pictures captured using the ultra-high-resolution optical coherence tomography (UHR-OCT). The central thickness of the corneal epithelium and total cornea was obtained using the slitlamp, and the results were compared with the thickness obtained using UHR-OCT. Results: High-quality ocular images and higher spatial resolutions were obtained using the slitlamp with extremely high magnifications and Movie Crop Function, rather than the traditional slitlamp. The structures and characteristics of the cornea, such as the normal epithelium, abnormal epithelium of corneal intraepithelial neoplasia, laser in situ keratomileusis interface, and contact lenses, were clearly visualized using this device. These features were confirmed by comparing the obtained images with those acquired using UHR-OCT. Moreover, the tear film debris on the ocular surface and the corneal nerve in the anterior corneal stroma were also visualized. The thicknesses of the corneal epithelium and total cornea were similar to that measured using UHR-OCT (P
AB - Purpose: To demonstrate the use of the slitlamp photography and videography with extremely high magnifications for visualizing structures of the anterior segment of the eye. Methods: A Canon 60D digital camera with Movie Crop Function was adapted into a Nikon FS-2 slitlamp to capture still images and video clips of the structures of the anterior segment of the eye. Images obtained using the slitlamp were tested for spatial resolution. The cornea of human eyes was imaged with the slitlamp, and the structures were compared with the pictures captured using the ultra-high-resolution optical coherence tomography (UHR-OCT). The central thickness of the corneal epithelium and total cornea was obtained using the slitlamp, and the results were compared with the thickness obtained using UHR-OCT. Results: High-quality ocular images and higher spatial resolutions were obtained using the slitlamp with extremely high magnifications and Movie Crop Function, rather than the traditional slitlamp. The structures and characteristics of the cornea, such as the normal epithelium, abnormal epithelium of corneal intraepithelial neoplasia, laser in situ keratomileusis interface, and contact lenses, were clearly visualized using this device. These features were confirmed by comparing the obtained images with those acquired using UHR-OCT. Moreover, the tear film debris on the ocular surface and the corneal nerve in the anterior corneal stroma were also visualized. The thicknesses of the corneal epithelium and total cornea were similar to that measured using UHR-OCT (P
KW - Corneal epithelial thickness
KW - High magnification
KW - Slitlamp biomicroscopy
KW - Ultra-high optical coherence topography
KW - Videography
UR - http://www.scopus.com/inward/record.url?scp=84946713825&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84946713825&partnerID=8YFLogxK
U2 - 10.1097/ICL.0000000000000148
DO - 10.1097/ICL.0000000000000148
M3 - Article
C2 - 26020484
AN - SCOPUS:84946713825
VL - 41
SP - 391
EP - 397
JO - Eye and Contact Lense
JF - Eye and Contact Lense
SN - 1542-2321
IS - 6
ER -