Skew orientation of biological samples Anacystis nidulans cyanobacteria and their fragments in a polymer matrix

Anacystis nidulans cyanobacteria and their fragments embedded in unstretched, uniaxial and skew (two axes of stretching forming an angle of 40°) stretched poly(vinyl alcohol) films have been investigated. Polarized absorption spectra for uniaxial and skew stretching samples were measured. Both unoriented and oriented samples were photographed under fluorescence microscope. In skew samples a high degree of cell orientation was reached. Skew deformation of polymer matrix compared to one axis stretching provides better band resolution in polarized absorption spectra of Anacystis nidulans samples. The shapes of absorption components measured in respect to the first and second axis of stretching are different which gives the opportunity to investigate position of various group of chlorophyll molecules in membrane.