Skew orientation of biological samples Anacystis nidulans cyanobacteria and their fragments in a polymer matrix

D. Frackowiak; A. Skibinski; B. Zelent; R. M. Leblanc

doi:10.1016/0006-291X(92)91213-A

Skew orientation of biological samples Anacystis nidulans cyanobacteria and their fragments in a polymer matrix

D. Frackowiak, A. Skibinski, B. Zelent, R. M. Leblanc

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Anacystis nidulans cyanobacteria and their fragments embedded in unstretched, uniaxial and skew (two axes of stretching forming an angle of 40°) stretched poly(vinyl alcohol) films have been investigated. Polarized absorption spectra for uniaxial and skew stretching samples were measured. Both unoriented and oriented samples were photographed under fluorescence microscope. In skew samples a high degree of cell orientation was reached. Skew deformation of polymer matrix compared to one axis stretching provides better band resolution in polarized absorption spectra of Anacystis nidulans samples. The shapes of absorption components measured in respect to the first and second axis of stretching are different which gives the opportunity to investigate position of various group of chlorophyll molecules in membrane.

Original language	English (US)
Pages (from-to)	441-447
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	184
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(92)91213-A
State	Published - Apr 15 1992
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Skew orientation of biological samples Anacystis nidulans cyanobacteria and their fragments in a polymer matrix",

abstract = "Anacystis nidulans cyanobacteria and their fragments embedded in unstretched, uniaxial and skew (two axes of stretching forming an angle of 40°) stretched poly(vinyl alcohol) films have been investigated. Polarized absorption spectra for uniaxial and skew stretching samples were measured. Both unoriented and oriented samples were photographed under fluorescence microscope. In skew samples a high degree of cell orientation was reached. Skew deformation of polymer matrix compared to one axis stretching provides better band resolution in polarized absorption spectra of Anacystis nidulans samples. The shapes of absorption components measured in respect to the first and second axis of stretching are different which gives the opportunity to investigate position of various group of chlorophyll molecules in membrane.",

author = "D. Frackowiak and A. Skibinski and B. Zelent and Leblanc, {R. M.}",

note = "Funding Information: ACKNOWLEDGMENTS. Roger M. Leblanc and Bogumil Zelent are grateful for financial support from the Natural Science and Enginering Research Council of Canada (NSERC) and Fonds pour la Formation de Chercheurs et I{\textquoteright}Aide a la Recherche (FCAR). Danuta Frackowiak and Aleksander Skibinski wish to thank for financial support from Polish Ministry of Education and Poznan Technical University. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1992",

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doi = "10.1016/0006-291X(92)91213-A",

language = "English (US)",

volume = "184",

pages = "441--447",

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T1 - Skew orientation of biological samples Anacystis nidulans cyanobacteria and their fragments in a polymer matrix

AU - Frackowiak, D.

AU - Skibinski, A.

AU - Zelent, B.

AU - Leblanc, R. M.

N1 - Funding Information: ACKNOWLEDGMENTS. Roger M. Leblanc and Bogumil Zelent are grateful for financial support from the Natural Science and Enginering Research Council of Canada (NSERC) and Fonds pour la Formation de Chercheurs et I’Aide a la Recherche (FCAR). Danuta Frackowiak and Aleksander Skibinski wish to thank for financial support from Polish Ministry of Education and Poznan Technical University. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1992/4/15

Y1 - 1992/4/15

N2 - Anacystis nidulans cyanobacteria and their fragments embedded in unstretched, uniaxial and skew (two axes of stretching forming an angle of 40°) stretched poly(vinyl alcohol) films have been investigated. Polarized absorption spectra for uniaxial and skew stretching samples were measured. Both unoriented and oriented samples were photographed under fluorescence microscope. In skew samples a high degree of cell orientation was reached. Skew deformation of polymer matrix compared to one axis stretching provides better band resolution in polarized absorption spectra of Anacystis nidulans samples. The shapes of absorption components measured in respect to the first and second axis of stretching are different which gives the opportunity to investigate position of various group of chlorophyll molecules in membrane.

AB - Anacystis nidulans cyanobacteria and their fragments embedded in unstretched, uniaxial and skew (two axes of stretching forming an angle of 40°) stretched poly(vinyl alcohol) films have been investigated. Polarized absorption spectra for uniaxial and skew stretching samples were measured. Both unoriented and oriented samples were photographed under fluorescence microscope. In skew samples a high degree of cell orientation was reached. Skew deformation of polymer matrix compared to one axis stretching provides better band resolution in polarized absorption spectra of Anacystis nidulans samples. The shapes of absorption components measured in respect to the first and second axis of stretching are different which gives the opportunity to investigate position of various group of chlorophyll molecules in membrane.

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JO - Biochemical and Biophysical Research Communications

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Skew orientation of biological samples Anacystis nidulans cyanobacteria and their fragments in a polymer matrix

Abstract

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