Skeletal myogenic differentiation of urine-derived stem cells and angiogenesis using microbeads loaded with growth factors

Guihua Liu, Rajesh A. Pareta, Rongpei Wu, Yingai Shi, Xiaobo Zhou, Hong Liu, Chunhua Deng, Xiangzhou Sun, Anthony Atala, Emmanuel C. Opara, Yuanyuan Zhang

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

To provide site-specific delivery and targeted release of growth factors to implanted urine-derived stem cells (USCs), we prepared microbeads of alginate containing growth factors. The growth factors included VEGF, IGF-1, FGF-1, PDGF, HGF and NGF. Radiolabeled growth factors were loaded separately and used to access the in vitro release from the microbeads with a gamma counter over 4 weeks. In vitro endothelial differentiation of USCs by the released VEGF from the microbeads in a separate experiment confirmed that the released growth factors from the microbeads were bioactive. USCs and microbeads were mixed with the collagen gel type 1 (2 mg/ml) and used for in vivo studies through subcutaneous injection into nude mice. Four weeks after subcutaneous injection, we found that grafted cell survival was improved and more cells expressed myogenic and endothelial cell transcripts and markers compared to controls. More vessel formation and innervations were observed in USCs combined with six growth factors cocktail incorporated in microbeads compared to controls. In conclusion, a combination of growth factors released locally from the alginate microbeads induced USCs to differentiate into a myogenic lineage, enhanced revascularization and innervation, and stimulated resident cell growth in vivo. This approach could potentially be used for cell therapy in the treatment of stress urinary incontinence.

Original languageEnglish
Pages (from-to)1311-1326
Number of pages16
JournalBiomaterials
Volume34
Issue number4
DOIs
StatePublished - Jan 1 2013
Externally publishedYes

Fingerprint

Stem cells
Microspheres
Intercellular Signaling Peptides and Proteins
Stem Cells
Urine
Alginate
Subcutaneous Injections
Vascular Endothelial Growth Factor A
Fibroblast Growth Factor 1
Stress Urinary Incontinence
Endothelial cells
Cell growth
Nerve Growth Factor
Cell- and Tissue-Based Therapy
Collagen Type I
Insulin-Like Growth Factor I
Collagen
Nude Mice
Cell Survival
Gels

Keywords

  • Alginate microbeads
  • Controlled release
  • Drug delivery
  • Growth factors
  • Stem cells

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Skeletal myogenic differentiation of urine-derived stem cells and angiogenesis using microbeads loaded with growth factors. / Liu, Guihua; Pareta, Rajesh A.; Wu, Rongpei; Shi, Yingai; Zhou, Xiaobo; Liu, Hong; Deng, Chunhua; Sun, Xiangzhou; Atala, Anthony; Opara, Emmanuel C.; Zhang, Yuanyuan.

In: Biomaterials, Vol. 34, No. 4, 01.01.2013, p. 1311-1326.

Research output: Contribution to journalArticle

Liu, G, Pareta, RA, Wu, R, Shi, Y, Zhou, X, Liu, H, Deng, C, Sun, X, Atala, A, Opara, EC & Zhang, Y 2013, 'Skeletal myogenic differentiation of urine-derived stem cells and angiogenesis using microbeads loaded with growth factors', Biomaterials, vol. 34, no. 4, pp. 1311-1326. https://doi.org/10.1016/j.biomaterials.2012.10.038
Liu, Guihua ; Pareta, Rajesh A. ; Wu, Rongpei ; Shi, Yingai ; Zhou, Xiaobo ; Liu, Hong ; Deng, Chunhua ; Sun, Xiangzhou ; Atala, Anthony ; Opara, Emmanuel C. ; Zhang, Yuanyuan. / Skeletal myogenic differentiation of urine-derived stem cells and angiogenesis using microbeads loaded with growth factors. In: Biomaterials. 2013 ; Vol. 34, No. 4. pp. 1311-1326.
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