SIVmac Expressing Hybrid Envelope Proteins Containing HIV-1 V3 and/or C4 Sequences is Not Competent for Replication

Frank Kirchhoff, Hilary G. Morrison, Michael G. Murray, Paul Rennert, Ronald C. Desrosiers

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The V3- and C4-coding regions in the envelope gene of the infectious, pathogenic SIVmac239 clone were replaced by the corresponding HIV-l sequences. Viral particles were obtained after transfection of COS-1 cells. Chimeric SIVmac constructs were not replication competent in the human T cell lines CEMx174, AA2, H9, and MT-4 or in primary cultures of rhesus monkey peripheral blood mononuclear cells. The lack of infectivity of the hybrid constructs was associated with inefficient proteolytic processing of the gp160env precursor. Unlike the modular nature of some proteins, gp 120 appears to be a highly ordered molecule whose function is dependent on the integration of many discontinuous, interactive regions.

Original languageEnglish (US)
Pages (from-to)309-313
Number of pages5
JournalAIDS research and human retroviruses
Volume10
Issue number3
DOIs
StatePublished - Mar 1994
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

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