SIVmac expressing hybrid envelope proteins containing HIV-1 V3 and/or C4 sequences is not competent for replication

F. Kirchhoff, H. G. Morrison, M. G. Murray, P. Rennert, Ronald Charles Desrosiers

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The V3- and C4-coding regions in the envelope gene of the infectious, pathogenic SIVmac239 clone were replaced by the corresponding HIV-1 sequences. Viral particles were obtained after transfection of COS-1 cells. Chimeric SIVmac constructs were not replication competent in the human T cell lines CEMx174, AA2, H9, and MT-4 or in primary cultures of rhesus monkey peripheral blood mononuclear cells. The lack of infectivity of the hybrid constructs was associated with inefficient proteolytic processing of the gp160(env) precursor. Unlike the modular nature of some proteins, gp120 appears to be a highly ordered molecule whose function is dependent on the integration of many discontinuous, interactive regions.

Original languageEnglish (US)
Pages (from-to)309-313
Number of pages5
JournalAIDS Research and Human Retroviruses
Volume10
Issue number3
StatePublished - 1994
Externally publishedYes

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COS Cells
Macaca mulatta
Virion
Transfection
HIV-1
Blood Cells
Clone Cells
T-Lymphocytes
Cell Line
Genes
Proteins

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

SIVmac expressing hybrid envelope proteins containing HIV-1 V3 and/or C4 sequences is not competent for replication. / Kirchhoff, F.; Morrison, H. G.; Murray, M. G.; Rennert, P.; Desrosiers, Ronald Charles.

In: AIDS Research and Human Retroviruses, Vol. 10, No. 3, 1994, p. 309-313.

Research output: Contribution to journalArticle

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