Abstract
The V3- and C4-coding regions in the envelope gene of the infectious, pathogenic SIVmac239 clone were replaced by the corresponding HIV-1 sequences. Viral particles were obtained after transfection of COS-1 cells. Chimeric SIVmac constructs were not replication competent in the human T cell lines CEMx174, AA2, H9, and MT-4 or in primary cultures of rhesus monkey peripheral blood mononuclear cells. The lack of infectivity of the hybrid constructs was associated with inefficient proteolytic processing of the gp160(env) precursor. Unlike the modular nature of some proteins, gp120 appears to be a highly ordered molecule whose function is dependent on the integration of many discontinuous, interactive regions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 309-313 |
| Number of pages | 5 |
| Journal | AIDS Research and Human Retroviruses |
| Volume | 10 |
| Issue number | 3 |
| State | Published - 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
- Virology
Cite this
SIVmac expressing hybrid envelope proteins containing HIV-1 V3 and/or C4 sequences is not competent for replication. / Kirchhoff, F.; Morrison, H. G.; Murray, M. G.; Rennert, P.; Desrosiers, Ronald Charles.
In: AIDS Research and Human Retroviruses, Vol. 10, No. 3, 1994, p. 309-313.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - SIVmac expressing hybrid envelope proteins containing HIV-1 V3 and/or C4 sequences is not competent for replication
AU - Kirchhoff, F.
AU - Morrison, H. G.
AU - Murray, M. G.
AU - Rennert, P.
AU - Desrosiers, Ronald Charles
PY - 1994
Y1 - 1994
N2 - The V3- and C4-coding regions in the envelope gene of the infectious, pathogenic SIVmac239 clone were replaced by the corresponding HIV-1 sequences. Viral particles were obtained after transfection of COS-1 cells. Chimeric SIVmac constructs were not replication competent in the human T cell lines CEMx174, AA2, H9, and MT-4 or in primary cultures of rhesus monkey peripheral blood mononuclear cells. The lack of infectivity of the hybrid constructs was associated with inefficient proteolytic processing of the gp160(env) precursor. Unlike the modular nature of some proteins, gp120 appears to be a highly ordered molecule whose function is dependent on the integration of many discontinuous, interactive regions.
AB - The V3- and C4-coding regions in the envelope gene of the infectious, pathogenic SIVmac239 clone were replaced by the corresponding HIV-1 sequences. Viral particles were obtained after transfection of COS-1 cells. Chimeric SIVmac constructs were not replication competent in the human T cell lines CEMx174, AA2, H9, and MT-4 or in primary cultures of rhesus monkey peripheral blood mononuclear cells. The lack of infectivity of the hybrid constructs was associated with inefficient proteolytic processing of the gp160(env) precursor. Unlike the modular nature of some proteins, gp120 appears to be a highly ordered molecule whose function is dependent on the integration of many discontinuous, interactive regions.
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UR - http://www.scopus.com/inward/citedby.url?scp=0028174555&partnerID=8YFLogxK
M3 - Article
C2 - 8018392
AN - SCOPUS:0028174555
VL - 10
SP - 309
EP - 313
JO - AIDS Research and Human Retroviruses
JF - AIDS Research and Human Retroviruses
SN - 0889-2229
IS - 3
ER -