@article{d89125fc592c4754a2fa9652f94a8adb,
title = "siRNA-directed inhibition of HIV-1 infection",
abstract = "RNA interference silences gene expression through short interfering 21-23-mer double-strand RNA segments that guide mRNA degradation in a sequence-specific fashion. Here we report that siRNAs inhibit virus production by targeting the mRNAs for either the HIV-1 cellular receptor CD4, the viral structural Gag protein or green fluorescence protein substituted for the Nef regulatory protein. siRNAs effectively inhibit pre- and/or post-integration infection events in the HIV-1 life cycle. Thus, siRNAs may have potential for therapeutic intervention in HIV-1 and other viral infections.",
author = "Novina, {Carl D.} and Murray, {Michael F.} and Dykxhoorn, {Derek M.} and Beresford, {Paul J.} and Jonathan Riess and Lee, {Sang Kyung} and Collman, {Ronald G.} and Judy Lieberman and Premlata Shankar and Sharp, {Phillip A.}",
note = "Funding Information: Acknowledgments We thank V. Fran{\c c}ois-Bongar{\c c}on for help and technical assistance; H. Cargill for help with Fig. 6; and J. Doench for helpful discussions and comments. The following reagents were obtained through the AIDS Research and Reference Reagent Program Division of AIDS, NIAID, NIH: HeLaT4+ and T4pMV7 were provided by R. Axel, pR7-GFP was provided by K. Page and M. Feinberg and Magi-CCR5 was provided by J. Overbaugh. This work was supported by grants NIH F32 AI10523 (to C.D.N.), NIH T32-GM07748 (to M.F.M.), NIH AI 35502 (to R.G.C.), NIH AI 45306 (to P.S.) and NIH MERIT grant R37-GM34277, NCI grant PO1-CA42063 and partially by NCI Cancer Center Support (Core) grant P30-CA14051 (to P.A.S.).",
year = "2002",
month = jul,
doi = "10.1038/nm725",
language = "English (US)",
volume = "8",
pages = "681--686",
journal = "Nature Medicine",
issn = "1078-8956",
publisher = "Nature Publishing Group",
number = "7",
}