siRNA-directed inhibition of HIV-1 infection

Carl D. Novina; Michael F. Murray; Derek M. Dykxhoorn; Paul J. Beresford; Jonathan Riess; Sang Kyung Lee; Ronald G. Collman; Judy Lieberman; Premlata Shankar; Phillip A. Sharp

doi:10.1038/nm725

siRNA-directed inhibition of HIV-1 infection

Carl D. Novina, Michael F. Murray, Derek M. Dykxhoorn, Paul J. Beresford, Jonathan Riess, Sang Kyung Lee, Ronald G. Collman, Judy Lieberman, Premlata Shankar, Phillip A. Sharp

Research output: Contribution to journal › Article

710 Scopus citations

Abstract

RNA interference silences gene expression through short interfering 21-23-mer double-strand RNA segments that guide mRNA degradation in a sequence-specific fashion. Here we report that siRNAs inhibit virus production by targeting the mRNAs for either the HIV-1 cellular receptor CD4, the viral structural Gag protein or green fluorescence protein substituted for the Nef regulatory protein. siRNAs effectively inhibit pre- and/or post-integration infection events in the HIV-1 life cycle. Thus, siRNAs may have potential for therapeutic intervention in HIV-1 and other viral infections.

Original language	English (US)
Pages (from-to)	681-686
Number of pages	6
Journal	Nature medicine
Volume	8
Issue number	7
DOIs	https://doi.org/10.1038/nm725
State	Published - Jul 2002
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1038/nm725

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Novina, C. D., Murray, M. F., Dykxhoorn, D. M., Beresford, P. J., Riess, J., Lee, S. K., Collman, R. G., Lieberman, J., Shankar, P., & Sharp, P. A. (2002). siRNA-directed inhibition of HIV-1 infection. Nature medicine, 8(7), 681-686. https://doi.org/10.1038/nm725

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title = "siRNA-directed inhibition of HIV-1 infection",

abstract = "RNA interference silences gene expression through short interfering 21-23-mer double-strand RNA segments that guide mRNA degradation in a sequence-specific fashion. Here we report that siRNAs inhibit virus production by targeting the mRNAs for either the HIV-1 cellular receptor CD4, the viral structural Gag protein or green fluorescence protein substituted for the Nef regulatory protein. siRNAs effectively inhibit pre- and/or post-integration infection events in the HIV-1 life cycle. Thus, siRNAs may have potential for therapeutic intervention in HIV-1 and other viral infections.",

author = "Novina, {Carl D.} and Murray, {Michael F.} and Dykxhoorn, {Derek M.} and Beresford, {Paul J.} and Jonathan Riess and Lee, {Sang Kyung} and Collman, {Ronald G.} and Judy Lieberman and Premlata Shankar and Sharp, {Phillip A.}",

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AU - Riess, Jonathan

AU - Lee, Sang Kyung

AU - Collman, Ronald G.

AU - Lieberman, Judy

AU - Shankar, Premlata

AU - Sharp, Phillip A.

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