Single step nanoplasmonic immunoassay for the measurement of protein biomarkers

Shradha Prabhulkar, Adam de la Zerda, Amit Paranjape, Richard M. Awdeh

Research output: Contribution to journalArticlepeer-review

Abstract

A nanoplasmonic biosensor for highly-sensitive, single-step detection of protein biomarkers is presented. The principle is based on the utilization of the optical scattering properties of gold nanorods (GNRs) conjugated to bio-recognition molecules. The nanoplasmonic properties of the GNRs were utilized to detect proteins using near-infrared light interferometry. We show that the antibody-conjugated GNRs can specifically bind to our model analyte, Glucose Transporter-1 (Glut-1). The signal intensity of back-scattered light from the GNRs bound after incubation, correlated well to the Glut-1 concentration as per the calibration curve. The detection range using this nanoplasmonic immunoassay ranges from 10 ng/mL to 1 ug/mL for Glut-1. The minimal detectable concentration based on the lowest discernable concentration from zero is 10 ng/mL. This nanoplasmonic immunoassay can act as a simple, selective, sensitive strategy for effective disease diagnosis. It offers advantages such as wide detection range, increased speed of analysis (due to fewer incubation/washing steps), and no label development as compared to traditional immunoassay techniques. Our future goal is to incorporate this detection strategy onto a microfluidic platform to be used as a point-of-care diagnostic tool.

Original languageEnglish (US)
Pages (from-to)77-88
Number of pages12
JournalBiosensors
Volume3
Issue number1
DOIs
StatePublished - Mar 2013

Keywords

  • Glucose transporter-1
  • Gold nanorods
  • Immunoassay
  • Optical coherence tomography
  • Protein biomarkers
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Clinical Biochemistry

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