Abstract
In this study, molecular beacons have been successfully transfected into living cells with high efficiency (80%∼90%) inside a microfluidic system. This will enable a sensitive and specific method to monitor gene expression dynamics in single living cells. Fluorescence conjugated transfection reagent was adopted to investigate the transfection dynamics of molecular beacons. The development of microfluidic systems has facilitated real-time monitoring of individual cells, which revealed the transfection dynamics of molecular beacons at the single cell level.
| Original language | English |
|---|---|
| Title of host publication | Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Japan Academic Association Inc |
| Pages | 969-971 |
| Number of pages | 3 |
| State | Published - Jan 1 2006 |
| Externally published | Yes |
| Event | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: Nov 5 2006 → Nov 9 2006 |
Other
| Other | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 |
|---|---|
| Country | Japan |
| City | Tokyo |
| Period | 11/5/06 → 11/9/06 |
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Keywords
- Gene expression dynamics
- Microfluidics
- Molecular beacon
- Single cells
- Transfection
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering
Cite this
Single cell transfection dynamics in a microfluidic system. / Li, Na; Wong, Pak Kin; Lin, James; Ho, Chih Ming.
Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, 2006. p. 969-971.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Single cell transfection dynamics in a microfluidic system
AU - Li, Na
AU - Wong, Pak Kin
AU - Lin, James
AU - Ho, Chih Ming
PY - 2006/1/1
Y1 - 2006/1/1
N2 - In this study, molecular beacons have been successfully transfected into living cells with high efficiency (80%∼90%) inside a microfluidic system. This will enable a sensitive and specific method to monitor gene expression dynamics in single living cells. Fluorescence conjugated transfection reagent was adopted to investigate the transfection dynamics of molecular beacons. The development of microfluidic systems has facilitated real-time monitoring of individual cells, which revealed the transfection dynamics of molecular beacons at the single cell level.
AB - In this study, molecular beacons have been successfully transfected into living cells with high efficiency (80%∼90%) inside a microfluidic system. This will enable a sensitive and specific method to monitor gene expression dynamics in single living cells. Fluorescence conjugated transfection reagent was adopted to investigate the transfection dynamics of molecular beacons. The development of microfluidic systems has facilitated real-time monitoring of individual cells, which revealed the transfection dynamics of molecular beacons at the single cell level.
KW - Gene expression dynamics
KW - Microfluidics
KW - Molecular beacon
KW - Single cells
KW - Transfection
UR - http://www.scopus.com/inward/record.url?scp=50149112832&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=50149112832&partnerID=8YFLogxK
M3 - Conference contribution
SP - 969
EP - 971
BT - Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Japan Academic Association Inc
ER -