Single-cell molecular profiling of all three components of the HPA axis reveals adrenal ABCB1 as a regulator of stress adaptation

Juan Pablo Lopez, Elena Brivio, Alice Santambrogio, Carlo De Donno, Aron Kos, Miriam Peters, Nicolas Rost, Darina Czamara, Tanja M. Brückl, Simone Roeh, Max L. Pöhlmann, Clara Engelhardt, Andrea Ressle, Rainer Stoffel, Alina Tontsch, Javier M. Villamizar, Martin Reincke, Anna Riester, Silviu Sbiera, Martin FassnachtHelen S. Mayberg, W. Edward Craighead, Boadie W. Dunlop, Charles B. Nemeroff, Mathias V. Schmidt, Elisabeth B. Binder, Fabian J. Theis, Felix Beuschlein, Cynthia L. Andoniadou, Alon Chen

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Chronic activation and dysregulation of the neuroendocrine stress response have severe physiological and psychological consequences, including the development of metabolic and stress-related psychiatric disorders. We provide the first unbiased, cell type-specific, molecular characterization of all three components of the hypothalamic-pituitary- adrenal axis, under baseline and chronic stress conditions. Among others, we identified a previously unreported subpopulation of Abcb1b+ cells involved in stress adaptation in the adrenal gland. We validated our findings in a mouse stress model, adrenal tissues from patients with Cushing's syndrome, adrenocortical cell lines, and peripheral cortisol and genotyping data from depressed patients. This extensive dataset provides a valuable resource for researchers and clinicians interested in the organism's nervous and endocrine responses to stress and the interplay between these tissues. Our findings raise the possibility that modulating ABCB1 function may be important in the development of treatment strategies for patients suffering from metabolic and stress-related psychiatric disorders.

Original languageEnglish (US)
Article numbereabe4497
JournalScience Advances
Volume7
Issue number5
DOIs
StatePublished - Jan 27 2021
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint

Dive into the research topics of 'Single-cell molecular profiling of all three components of the HPA axis reveals adrenal ABCB1 as a regulator of stress adaptation'. Together they form a unique fingerprint.

Cite this