Simultaneous quantitation of quinidine, procainamide, and N-acetylprocainamide in serum by gas-liquid chromatography with a nitrogen-phosphorus selective detector

K. M. Kessler, P. Ho-Tung, B. Steele, J. Silver, A. Pickoff, S. Narayanan, R. J. Myerburg

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The authors describe a single-run method for quantitating quinidine, procainamide, and N-acetylprocainamide, involving gas-liquid chromatography with a nitrogen-phosphorus selective detector. Within-run precision (CV) was 3% (x̄ = 2 mg/L, n = 20), 6.9% (x̄ = 4 mg/L, n = 10), and 1.5% (x̄ = 8 mg/L, n = 8) for quinidine; 7.7% (x̄ = 4 mg/L, n = 14), 1.6% (x̄ = 8 mg/L, n = 16), and 2.3% (x̄ = 12 mg/L, n = 12) for procainamide; and 6.3% (x̄ = 5 mg/L, n = 6), 3.6% (x̄ = 10 mg/L, n = 20), and 4.0% (x̄ = 20 mg/L, n = 10) for N-acetylprocainamide. Between-run precision was 3.0% (x̄ = 2 mg/L, n = 20), 7.0% (x̄ = 4 mg/L, n = 9), and 2.8% (x̄ = 8 mg/L, n = 9) for quinidine; 4.7% (x̄ = 4 mg/L, n = 10), 3.3% (x̄ = 8 mg/L, n = 20), and 1.9% (x̄ = 12 mg/L, n = 10) for procainamide; and 9.3% (x̄ = 5 mg/L, n = 6), 4.3% (x̄ = 10 mg/L, n = 20), and 3.8% (x̄ = 20 mg/L, n = 10) for N-acetylprocainamide. Tube stoppers that contain a rubber plasticizer interfere with the technique. Clinical application and correlation with drug concentrations by this technique are discussed.

Original languageEnglish (US)
Pages (from-to)1187-1190
Number of pages4
JournalUnknown Journal
Volume28
Issue number5
DOIs
StatePublished - 1982
Externally publishedYes

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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