Simultaneous quantitation of plasma doxorubicin and prochlorperazine content by high-performance liquid chromatography

Caihong Mou, Neil Ganju, Kasi S. Sridhar, Awtar Krishan

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

A high-performance liquid chromatographic method has been developed and tested for simultaneous extraction, elution and determination of doxorubicin and prochlorperazine content in human plasma samples. The procedure consists of extraction through a conditioned C18 solid-phase extraction cartridge, elution from a Spherisorb C8 reversed-phase column by an isocratic mobile phase (60% acetonitrile, 15% methanol and 25% buffer) followed by detection with electrochemical and fluorescence detectors. Recovery of doxorubicin and prochlorperazine from pooled human plasma samples (n=3) containing 100 ng/ml of the two drugs was 77.8 ± 3.5% and 89.1 ± 6.0%, respectively. The lower limits of quantitation for doxorubicin and prochlorperazine in plasma samples were 6.25 ng/ml and 10 ng/ml, respectively. A linear calibration curve was obtained for up to 2 μg/ml of doxorubicin and prochlorperazine. This combination method may be of particular value in clinical studies where phenothiazines such as prochlorperazine are used to enhance retention of doxorubicin in drug resistant tumor cells.

Original languageEnglish (US)
Pages (from-to)217-224
Number of pages8
JournalJournal of Chromatography B: Biomedical Applications
Volume703
Issue number1-2
DOIs
StatePublished - Dec 5 1997

Keywords

  • Doxorubicin
  • Prochlorperazine

ASJC Scopus subject areas

  • Chemistry(all)

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