Simultaneous positive and purifying selection on overlapping reading frames of the tat and vpr genes of simian immunodeficiency virus

A. L. Hughes, K. Westover, J. Da Silva, D. H. O'Connor, David Watkins

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Tat-specific cytotoxic T cells have previously been shown to exert positive Darwinian selection favoring amino acid replacements of an epitope of simian immunodeficiency virus (SIV). The region of the tat gene encoding this epitope falls within a region of overlap between the tat and vpr reading frames, and nonsynonymous nucleotide substitutions in the tat reading frame were found to occur disproportionately in such a way as to cause synonymous changes in the vpr reading frame. Comparison of published complete SIV genomes showed Tat to be the least conserved at the amino acid level of nine proteins encoded by the virus, while Vpr was one of the most conserved. Numerous parallel amino acid changes occurred within the Tat epitope independently in different monkeys, and purifying selection on the vpr reading frame, by limiting acceptable nonsynonymous substitutions in the tat reading frame, evidently has enhanced the probability of parallel evolution.

Original languageEnglish
Pages (from-to)7966-7972
Number of pages7
JournalJournal of Virology
Volume75
Issue number17
DOIs
StatePublished - Aug 23 2001
Externally publishedYes

Fingerprint

tat Genes
vpr Genes
Simian immunodeficiency virus
Simian Immunodeficiency Virus
Reading Frames
epitopes
Epitopes
genes
Amino Acids
amino acids
parallel evolution
Haplorhini
monkeys
Nucleotides
T-lymphocytes
nucleotides
Genome
Viruses
T-Lymphocytes
viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Simultaneous positive and purifying selection on overlapping reading frames of the tat and vpr genes of simian immunodeficiency virus. / Hughes, A. L.; Westover, K.; Da Silva, J.; O'Connor, D. H.; Watkins, David.

In: Journal of Virology, Vol. 75, No. 17, 23.08.2001, p. 7966-7972.

Research output: Contribution to journalArticle

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