Simultaneous measurement of salicylate hydroxylation and glutamate release in the penumbral cortex following transient middle cerebral artery occlusion in rats

Tadashi Morimoto, Mordecai Y.T. Globus, Raul Busto, Elena Martinez, Myron D. Ginsberg

Research output: Contribution to journalArticle

125 Scopus citations

Abstract

Using the microdialysis technique and laser-Doppler flowmetry, we performed simultaneous measurement of salicylate hydroxylation and glutamate release along with local CBF in the ischemic penumbral cortex of rat brain subjected to normothermic transient middle cerebral artery (MCA) occlusion. Cortical CBF fell to 24 ± 11% (mean ± SD) during ischemia and recovered to 84 ± 16% during reperfusion. Extracellular glutamate levels increased by 6.5-fold above baseline 10 min following MCA occlusion but subsequently returned to near baseline levels in spite of the persistent ischemia. Increase in 2,3- and 2,5-dihydroxybenzoic acid (DHBA) concentrations in the microdialysis perfusate was confirmed during both ischemia and reperfusion phase. Although the temporal profile and amount of salicylate hydroxylation were heterogeneous among individual animals, integrated 2.3-DHBA concentrations during reperfusion were correlated positively with integrated glutamate concentrations during ischemia and negatively with mean postischemic CBF. These relationships suggest a possible association of the enhanced production of 2,3-DHBA during reperfusion with larger amounts of intraischemic glutamate release and lower levels of postischemic CBF.

Original languageEnglish (US)
Pages (from-to)92-99
Number of pages8
JournalJournal of Cerebral Blood Flow and Metabolism
Volume16
Issue number1
DOIs
StatePublished - Jan 1 1996

Keywords

  • Excitotoxicity
  • Hydroxyl radical
  • Microdialysis
  • Salicylate trapping
  • Transient focal ischemia

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

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