Simultaneous measurement of cerebral blood flow and mRNA signals

Pixel-based inter-modality correlational analysis

Weizhao Zhao, Raul Busto, Jessie Truettner, Myron Ginsberg

Research output: Contribution to journalArticle

Abstract

The analysis of pixel-based relationships between local cerebral blood flow (LCBF) and mRNA expression can reveal important insights into brain function. Traditionally, LCBF and in situ hybridization studies for genes of interest have been analyzed in separate series. To overcome this limitation and to increase the power of statistical analysis, this study focused on developing a double-label method to measure local cerebral blood flow (LCBF) and gene expressions simultaneously by means of a dual-autoradiography procedure. A 14C-iodoantipyrine autoradiographic LCBF study was first performed. Serial brain sections (12 in this study) were obtained at multiple coronal levels and were processed in the conventional manner to yield quantitative LCBF images. Two replicate sections at each bregma level were then used for in situ hybridization. To eliminate the 14C-iodoantipyrine from these sections, a chloroform-washout procedure was first performed. The sections were then processed for in situ hybridization autoradiography for the probes of interest. This method was tested in Wistar rats subjected to 12 min of global forebrain ischemia by two-vessel occlusion plus hypotension, followed by 2 or 6 h of reperfusion (n=4-6 per group). LCBF and in situ hybridization images for heat shock protein 70 (HSP70) were generated for each rat, aligned by disparity analysis, and analyzed on a pixel-by-pixel basis. This method yielded detailed inter-modality correlation between LCBF and HSP70 mRNA expressions. The advantages of this method include reducing the number of experimental animals by one-half; and providing accurate pixel-based correlations between different modalities in the same animals, thus enabling paired statistical analyses. This method can be extended to permit correlation of LCBF with the expression of multiple genes of interest.

Original languageEnglish
Pages (from-to)161-170
Number of pages10
JournalJournal of Neuroscience Methods
Volume108
Issue number2
DOIs
StatePublished - Jul 30 2001

Fingerprint

Cerebrovascular Circulation
Messenger RNA
In Situ Hybridization
HSP70 Heat-Shock Proteins
Autoradiography
Gene Expression
Brain
Chloroform
Prosencephalon
Hypotension
Reperfusion
Wistar Rats
Ischemia

Keywords

  • Autoradiography
  • Cerebral blood flow
  • Image-processing
  • In situ hybridization
  • mRNA expression

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Simultaneous measurement of cerebral blood flow and mRNA signals : Pixel-based inter-modality correlational analysis. / Zhao, Weizhao; Busto, Raul; Truettner, Jessie; Ginsberg, Myron.

In: Journal of Neuroscience Methods, Vol. 108, No. 2, 30.07.2001, p. 161-170.

Research output: Contribution to journalArticle

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