Simultaneous cytokinetic measurement of aneuploid tumors and associated diploid cells following continuous labelling with chlorodeoxyuridine

R. Allen White, Alan Pollack, Nicholas H.A. Terry

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Cells from a murine tumor, MCa-K were continuously labelled with thymidine analogue chlorodeoxyuridine (CldUrd) and analyzed by bivariate cytometry in order to measure the growth fraction (GF) and potential doubling time (T(pot)) of both the DNA-aneu ploidtumor cells and the associated DNA-diploid cells. MCa-K has a DNA dex of 1.7, rendering two, partially, lapping, populations observable with labelled and unlabelled cells in population. The data from these tumors may be divided into three regions of fering DNA content, with one region taining a pure DNA-diploid population second region with both cell types, and a third region including only DNA-aneuploid cells. Equations are presented characterize the fractions of labelling time and cell type, thereby per mitting estimation of the proliferative properties of the populations. These equations include the possibility that DNA-aneuploid cells cease cycling both in G(1) and in S phase to account for observed numbers of unlabelled cells with S phase contents. The estimated value of T(pot) of the DNA-diploid cells is 126.0 h with a GF of 42%, while that of DNA-aneuploid cells is 36.9 h with a GF 69%. It is also estimated that between 2% and 6% of all DNA-aneuploid cells starting DNA synthesis cease cycling, lead to 25% of the cells having an S-phase DNA content being noncycling.

Original languageEnglish (US)
Pages (from-to)311-319
Number of pages9
JournalCytometry
Volume15
Issue number4
DOIs
StatePublished - Apr 1 1994
Externally publishedYes

    Fingerprint

Keywords

  • Bivariate flow cytometry
  • Cell kinetics
  • Chlorodeoxyuridine
  • Mathematical models
  • Normal tissues
  • Quiescence

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Endocrinology
  • Hematology
  • Pathology and Forensic Medicine

Cite this