Simultaneous analysis of tumor and stromal gene expression profiles from xenograft models

Elizabeth Iorns, Jennifer Clarke, Toby Ward, Sonja Dean, Marc E Lippman

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Identifying the gene expression alterations that occur in both the tumor and stroma is essential to understanding tumor biology. We have developed a dual-species microarray analysis method that allows the dissection of both tumor and stromal gene expression profiles from xenograft models, based on limited interspecies cross-hybridization on Illumina gene expression beadchips. This methodology allows for simultaneous genome-wide analysis of gene expression profiles of both tumor cells and the associated stromal tissue.

Original languageEnglish
Pages (from-to)321-324
Number of pages4
JournalBreast Cancer Research and Treatment
Volume131
Issue number1
DOIs
StatePublished - Jan 1 2012

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Keywords

  • Cross-hybridization
  • Dual-species
  • Gene expression profile
  • Microarray
  • Tumor microenvironment
  • Tumor stroma
  • Xenograft model

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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