Simple Determination of Microbial Protein in Rumen Liquor

H. P.S. Makkar, O. P. Sharma, R. K. Dawra, S. S. Negi

Research output: Contribution to journalArticlepeer-review

85 Scopus citations


A method for microbial protein determination based on Lowry's assay was devised. Differential centrifiguration was used to separate bacterial cells from rumen liquor. Proteins from cells were released by suspending in .25 N sodium hydroxide and heating in boiling water bath for 10 min. Protein by the Foin phenol method. The method is simple, accurate, reproducible, sensitive, specific, brief, and can be performed with small amount of rumen liquor (5 ml). A large number of samples can be handled conveniently.

Original languageEnglish (US)
Pages (from-to)2170-2173
Number of pages4
JournalJournal of dairy science
Issue number11
StatePublished - 1982
Externally publishedYes

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics


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