Simple Determination of Microbial Protein in Rumen Liquor

H. P.S. Makkar, O. P. Sharma, Rajinder Dawra, S. S. Negi

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

A method for microbial protein determination based on Lowry's assay was devised. Differential centrifiguration was used to separate bacterial cells from rumen liquor. Proteins from cells were released by suspending in .25 N sodium hydroxide and heating in boiling water bath for 10 min. Protein by the Foin phenol method. The method is simple, accurate, reproducible, sensitive, specific, brief, and can be performed with small amount of rumen liquor (5 ml). A large number of samples can be handled conveniently.

Original languageEnglish (US)
Pages (from-to)2170-2173
Number of pages4
JournalJournal of Dairy Science
Volume65
Issue number11
DOIs
StatePublished - Jan 1 1982
Externally publishedYes

Fingerprint

microbial proteins
Rumen
rumen fluids
Sodium Hydroxide
Proteins
sodium hydroxide
Phenol
boiling
Baths
Heating
phenol
proteins
methodology
cells
heat
Water
assays
water
sampling

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Simple Determination of Microbial Protein in Rumen Liquor. / Makkar, H. P.S.; Sharma, O. P.; Dawra, Rajinder; Negi, S. S.

In: Journal of Dairy Science, Vol. 65, No. 11, 01.01.1982, p. 2170-2173.

Research output: Contribution to journalArticle

Makkar, H. P.S. ; Sharma, O. P. ; Dawra, Rajinder ; Negi, S. S. / Simple Determination of Microbial Protein in Rumen Liquor. In: Journal of Dairy Science. 1982 ; Vol. 65, No. 11. pp. 2170-2173.
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