Simple and rapid method to isolate and culture follicular papillae from human scalp hair follicles

Markus Magerl, Söbia Kauser, Ralf Paus, Desmond J. Tobin

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Study of the involvement of the hair follicle papilla in hair growth regulation was greatly facilitated by the isolation and cultivation of this tiny cluster of fibroblast-like cells in the rat vibrissae and in the human hair follicle. While isolation of the hair follicle papilla from the former is relatively straightforward, the current method to isolate the much smaller human hair follicle requires significant skill. Thus, the routine initiation of primary cultures of human scalp hair follicle papilla cells requires significant training, time, and commitment. In an attempt to simplify hair follicle papilla cell culture methodology for new laboratory personnel, we have made significant refinements to the current method. Our method requires only two simple manipulations to isolate hair follicle papilla from intact isolated hair follicles. This very rapid and easy method isolates clean and intact hair follicle papillae. Together with their attachment via scratching to the growth surface, the isolation and cultivation of this important hair follicle component can now be achieved easily by the laboratory newcomer. The method relies for its simplicity on the removal of the hair follicle papilla from the outside of the intact hair follicle rather than via internal manipulations from within the hair follicle.

Original languageEnglish (US)
Pages (from-to)381-385
Number of pages5
JournalExperimental Dermatology
Volume11
Issue number4
DOIs
StatePublished - Aug 1 2002
Externally publishedYes

Fingerprint

Hair Follicle
Scalp
Fibroblasts
Cell culture
Rats
Cells
Personnel
Laboratory Personnel
Vibrissae
Growth
Hair
Cell Culture Techniques

Keywords

  • Cell culture
  • Dermal papilla
  • Hair follicle
  • Hair follicle papilla
  • Microdissection

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Simple and rapid method to isolate and culture follicular papillae from human scalp hair follicles. / Magerl, Markus; Kauser, Söbia; Paus, Ralf; Tobin, Desmond J.

In: Experimental Dermatology, Vol. 11, No. 4, 01.08.2002, p. 381-385.

Research output: Contribution to journalArticle

Magerl, Markus ; Kauser, Söbia ; Paus, Ralf ; Tobin, Desmond J. / Simple and rapid method to isolate and culture follicular papillae from human scalp hair follicles. In: Experimental Dermatology. 2002 ; Vol. 11, No. 4. pp. 381-385.
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