Significance of N-methyl-D-aspartate (NMDA) receptor-mediated signaling in human keratinocytes

Walter K. Nahm, Benjamin D. Philpot, Michelle M. Adams, Evangelos V Badiavas, Linda H. Zhou, Janet Butmarc, Mark F. Bear, Vincent Falanga

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Increasing data suggest that glutamate might act as a cell-signaling molecule in non-neuronal tissues such as the skin. Here we demonstrate the presence of functional N-methyl-D-aspartate (NMDA)-type glutamate receptors in human keratinocytes. NMDA receptor expression strongly reflects the degree of cell-to-cell contact. Wounding polarizes the expression of NMDA receptors in keratinocytes involved in re-epithelialization, and the process of re-epithelialization is inhibited by NMDA receptor activation. We also demonstrate that squamous cell carcinomas lack NMDA receptors. Our data suggest that Ca2+ entry through NMDA receptors influences the cycle of keratinocyte proliferation, differentiation, and migration during epithelialization. Moreover, NMDA receptor activation might play a role in contact-mediated inhibition of growth, a process that is absent during neoplastic pathology. This receptor may serve as a pharmacological target for modulating keratinocyte behavior and treating cutaneous disorders.

Original languageEnglish
Pages (from-to)309-317
Number of pages9
JournalJournal of Cellular Physiology
Volume200
Issue number2
DOIs
StatePublished - Aug 1 2004
Externally publishedYes

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N-Methyl-D-Aspartate Receptors
Keratinocytes
Re-Epithelialization
Chemical activation
Cell signaling
Contact Inhibition
Glutamate Receptors
Pathology
Skin
N-Methylaspartate
Glutamic Acid
Squamous Cell Carcinoma
Tissue
Pharmacology
Molecules
Growth

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Significance of N-methyl-D-aspartate (NMDA) receptor-mediated signaling in human keratinocytes. / Nahm, Walter K.; Philpot, Benjamin D.; Adams, Michelle M.; Badiavas, Evangelos V; Zhou, Linda H.; Butmarc, Janet; Bear, Mark F.; Falanga, Vincent.

In: Journal of Cellular Physiology, Vol. 200, No. 2, 01.08.2004, p. 309-317.

Research output: Contribution to journalArticle

Nahm, WK, Philpot, BD, Adams, MM, Badiavas, EV, Zhou, LH, Butmarc, J, Bear, MF & Falanga, V 2004, 'Significance of N-methyl-D-aspartate (NMDA) receptor-mediated signaling in human keratinocytes', Journal of Cellular Physiology, vol. 200, no. 2, pp. 309-317. https://doi.org/10.1002/jcp.20010
Nahm, Walter K. ; Philpot, Benjamin D. ; Adams, Michelle M. ; Badiavas, Evangelos V ; Zhou, Linda H. ; Butmarc, Janet ; Bear, Mark F. ; Falanga, Vincent. / Significance of N-methyl-D-aspartate (NMDA) receptor-mediated signaling in human keratinocytes. In: Journal of Cellular Physiology. 2004 ; Vol. 200, No. 2. pp. 309-317.
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