Signal requirements in the step-wise functional maturation of cytotoxic T lymphocytes

Gianni Gromo, Robin L. Geller, Luca Inverardi, Fritz H. Bach

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Abstract

The generation of effector cytotoxic T lymphocytes from resting precursors proceeds through a series of steps in a pathway that, in aggregate, involves both proliferation and development of cytotoxicity. To understand the relationship of the various signals (mitogens and/or lymphokines) that bring about progress along this pathway, it is desirable to define a series of 'minimal signals', each of which stimulates the cell to proceed to a further stage in this process of differentiation. Stimulation of lymphocytes with two different monoclonal antibodies directed against the CD2 surface molecule induces a proliferative response; we report here that both CD4+ and CD8+ cells proliferate in response to such a stimulus but do not develop cytotoxicity. Addition of recombinant γ-interferon (rIFN-γ) or recombinant IL-2 (rIL-2) to the activated cells leads to acquisition of cytotoxic status by the CD8+ cells but not the CD4+ cells. The availability, in addition to precursors and effectors, of an apparently intermediate stage in the form of proliferating CD8+ cells that are non-cytotoxic should facilitate both cellular and molecular studies of this maturation pathway; the differences between CD4+ and CD8+ cells in development of cytotoxicity under these experimental conditions is a valuable model for understanding differentiation of T lymphocytes.

Original languageEnglish (US)
Pages (from-to)424-426
Number of pages3
JournalNature
Volume327
Issue number6121
DOIs
StatePublished - Jan 1 1987

ASJC Scopus subject areas

  • General

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    Gromo, G., Geller, R. L., Inverardi, L., & Bach, F. H. (1987). Signal requirements in the step-wise functional maturation of cytotoxic T lymphocytes. Nature, 327(6121), 424-426. https://doi.org/10.1038/327424a0